BMRB Entry 34476

Title:
Solution structure of antimicrobial peptide, crabrolin Plus in the presence of Lipopolysaccharide
Deposition date:
2020-01-13
Original release date:
2020-07-20
Authors:
Cantini, F.; Bouchemal, N.; Savarin, P.; Sette, M.
Citation:

Citation: Cantini, F.; Luzi, C.; Bouchemal, N.; Savarin, P.; Bozzi, A.; Sette, M.. "Effect of positive charges in the structural interaction of crabrolin isoforms with lipopolysaccharide."  J. Pept. Sci. ., e3271-e3271 (2020).
PubMed: 32585759

Assembly members:

Assembly members:
entity_1, polymer, 13 residues, 1571.048 Da.

Natural source:

Natural source:   Common Name: European hornet   Taxonomy ID: 7445   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Vespa crabro

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: FLPKILRKIVRAL

Data sets:
Data typeCount
13C chemical shifts44
1H chemical shifts118

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 13 residues - 1571.048 Da.

1   PHELEUPROLYSILELEUARGLYSILEVAL
2   ARGALALEU

Samples:

sample_1: Crabrolin Plus, mutant of Crabrolin peptide 0.87 mM; Lipopolysaccharide 14.6 uM

sample_conditions_1: ionic strength: 125 mM; pH: 6.1; pressure: 760 mmHg; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1isotropicsample_conditions_1
2D TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller and Wuthrich - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

Sparky, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE 600 MHz