BMRB Entry 34443

Name: Timeless couples G quadruplex detection with processing by DDX11 during DNA replication
Published: 2020-06-26

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PDB ID: 6taz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34443
MolProbity Validation Chart

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NMR-STAR v3 text file.
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Timeless couples G quadruplex detection with processing by DDX11 during DNA replication

Deposition date:

2019-10-31

Original release date:

2020-06-26

Authors:

Lerner Koch, L.; Holzer, S.; Kilkenny, M.; Murat, P.; Svikovic, S.; Schiavone, D.; Bittleston, A.; Maman, J.; Branzei, D.; Stott, K.; Pellegrini, L.; Sale, E.

Citation:

Citation: Lerner, Leticia; Holzer, Sandro; Kilkenny, Mairi; Svikovic, Sasa; Murat, Pierre; Schiavone, Davide; Eldridge, Cara; Bittleston, Alice; Maman, Joseph; Branzei, Dana; Stott, Katherine; Pellegrini, Luca; Sale, Julian. "Timeless couples G quadruplex detection with processing by DDX11 during DNA replication" Embo J. ., e104185-e104185 (2020).
PubMed: 32705708

Assembly members:

Assembly members:
entity_1, polymer, 141 residues, 16359.771 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: DPSRRAPTWSPEEEAHLREL YLANKDVEGQDVVEAILAHL NTVPRTRKQIIHHLVQMGLA DSVKDFQRKGTHIVLWTGDQ ELELQRLFEEFRDSDDVLGH IMKNITAKRSRARIVDKLLA LGLVAERRELYKKRQKKLAS S

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	638
15N chemical shifts	151
1H chemical shifts	1041

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 141 residues - 16359.771 Da.

1

ASP

PRO

SER

ARG

ALA

PRO

THR

TRP

SER

2

PRO

GLU

ALA

HIS

LEU

ARG

GLU

LEU

3

TYR

LEU

ALA

ASN

LYS

ASP

VAL

GLU

GLY

GLN

4

ASP

VAL

GLU

ALA

ILE

LEU

ALA

HIS

LEU

5

ASN

THR

VAL

PRO

ARG

THR

ARG

LYS

GLN

ILE

6

ILE

HIS

LEU

VAL

GLN

MET

GLY

LEU

ALA

7

ASP

SER

VAL

LYS

ASP

PHE

GLN

ARG

LYS

GLY

8

THR

HIS

ILE

VAL

LEU

TRP

THR

GLY

ASP

GLN

9

GLU

LEU

GLU

LEU

GLN

ARG

LEU

PHE

GLU

10

PHE

ARG

ASP

SER

ASP

VAL

LEU

GLY

HIS

11

ILE

MET

LYS

ASN

ILE

THR

ALA

LYS

ARG

SER

12

ARG

ALA

ARG

ILE

VAL

ASP

LYS

LEU

ALA

13

LEU

GLY

LEU

VAL

ALA

GLU

ARG

GLU

LEU

14

TYR

LYS

ARG

GLN

LYS

LEU

ALA

SER

15

SER

Samples:

sample_1: TimDBD, [U-13C; U-15N], 0.5 mM; sodium phosphate, NA, 10 mM; potassium chloride, NA, 40 mM; EDTA, NA, 0.5 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

ARIA, Linge, O'Donoghue and Nilges - structure calculation

CcpNmr Analysis, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE III 800 MHz
Bruker AVANCE 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks