BMRB Entry 34291

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PDB ID: 6gvu
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34291
MolProbity Validation Chart

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Title:
NMR structure of the DNA-bound helix bundle domain from the functional pRN1 primase
Deposition date:
2018-06-21
Original release date:
2018-12-19
Authors:
Boudet, J.; Lipps, G.; Allain, F.
Citation:

Citation: Boudet, J.; Devillier, J.; Wiegand, T.; Salmon, L.; Meier, B.; Lipps, G.; Allain, F.. "A Small Helical Bundle Prepares Primer Synthesis by Binding Two Nucleotides that Enhance Sequence-Specific Recognition of the DNA Template"  Cell 176, 154-166 (2019).
PubMed: 30595448

Assembly members:

Assembly members:
entity_1, polymer, 9 residues, 2706.785 Da.
entity_2, polymer, 115 residues, 13528.738 Da.

Natural source:

Natural source:   Common Name: Sulfolobus islandicus   Taxonomy ID: 43080   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus islandicus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: CTGTGCTCA
entity_2: TVVEFEELRKELVKRDSGKP VEKIKEEICTKSPPKLIKEI ICENKTYADVNIDRSRGDWH VILYLMKHGVTDPDKILELL PRDSKAKENEKWNTQKYFVI TLSKAWSVVKKYLEA

Data sets:
Data typeCount
13C chemical shifts303
15N chemical shifts104
1H chemical shifts779

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 9 residues - 2706.785 Da.

1   DCDTDGDTDGDCDTDCDA

Entity 2, entity_2 115 residues - 13528.738 Da.

1   THRVALVALGLUPHEGLUGLULEUARGLYS
2   GLULEUVALLYSARGASPSERGLYLYSPRO
3   VALGLULYSILELYSGLUGLUILECYSTHR
4   LYSSERPROPROLYSLEUILELYSGLUILE
5   ILECYSGLUASNLYSTHRTYRALAASPVAL
6   ASNILEASPARGSERARGGLYASPTRPHIS
7   VALILELEUTYRLEUMETLYSHISGLYVAL
8   THRASPPROASPLYSILELEUGLULEULEU
9   PROARGASPSERLYSALALYSGLUASNGLU
10   LYSTRPASNTHRGLNLYSTYRPHEVALILE
11   THRLEUSERLYSALATRPSERVALVALLYS
12   LYSTYRLEUGLUALA

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