BMRB Entry 34255

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PDB ID: 6g5r
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34255
MolProbity Validation Chart

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Title:
Structure of the UB2H domain of E.coli PBP1B in complex with LpoB
Deposition date:
2018-03-29
Original release date:
2019-03-14
Authors:
Simorre, J.; Maya Martinez, R.; Bougault, C.
Citation:

Citation: Egan, A.; Maya-Martinez, R.; Ayala, I.; Bougault, C.; Banzhaf, M.; Breukink, E.; Vollmer, W.; Simorre, J.. "Induced conformational changes activate the peptidoglycan synthase PBP1B."  Mol. Microbiol. 110, 335-356 (2018).
PubMed: 30044025

Assembly members:

Assembly members:
entity_1, polymer, 114 residues, 13183.969 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 83333   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MGRMVNLEPDMTISKNEMVK LLEATQYRQVSKMTRPGEFT VQANSIEMIRRPFDFPDSKE GQVRARLTFDGDHLATIVNM ENNRQFGFFRLDPR

Data sets:
Data typeCount
13C chemical shifts363
15N chemical shifts72
1H chemical shifts499

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 114 residues - 13183.969 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METGLYARGMETVALASNLEUGLUPROASP
4   METTHRILESERLYSASNGLUMETVALLYS
5   LEULEUGLUALATHRGLNTYRARGGLNVAL
6   SERLYSMETTHRARGPROGLYGLUPHETHR
7   VALGLNALAASNSERILEGLUMETILEARG
8   ARGPROPHEASPPHEPROASPSERLYSGLU
9   GLYGLNVALARGALAARGLEUTHRPHEASP
10   GLYASPHISLEUALATHRILEVALASNMET
11   GLUASNASNARGGLNPHEGLYPHEPHEARG
12   LEUASPPROARG

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