BMRB Entry 34234

Title:
E.coli Sigma factor S (RpoS) Region 4
Deposition date:
2018-01-17
Original release date:
2018-06-01
Authors:
Liu, B.; Matthews, S.
Citation:

Citation: Tabib-Salazar, A.; Liu, B.; Barker, D.; Burchell, L.; Qimron, U.; Matthews, S.; Wigneshweraraj, S.. "T7 phage factor required for managing RpoS inEscherichia coli."  Proc. Natl. Acad. Sci. U.S.A. 115, E5353-E5362 (2018).
PubMed: 29789383

Assembly members:

Assembly members:
entity_1, polymer, 86 residues, 10041.383 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 83333   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts390
15N chemical shifts96
1H chemical shifts647

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 86 residues - 10041.383 Da.

1   GLYPROGLUASPTHRTHRGLNASPASPASP
2   METLYSGLNSERILEVALLYSTRPLEUPHE
3   GLULEUASNALALYSGLNARGGLUVALLEU
4   ALAARGARGPHEGLYLEULEUGLYTYRGLU
5   ALAALATHRLEUGLUASPVALGLYARGGLU
6   ILEGLYLEUTHRARGGLUARGVALARGGLN
7   ILEGLNVALGLUGLYLEUARGARGLEUARG
8   GLUILELEUGLNTHRGLNGLYLEUASNILE
9   GLUALALEUPHEARGGLU

Samples:

sample_1: E.coli Sigma factor S region 4, [U-99% 13C; U-99% 15N], 1 ± 0.1 mM

sample_conditions_1: ionic strength: 350 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

ARIA, Linge, O'Donoghue and Nilges - structure calculation

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Advance HD III 600 MHz
  • Bruker Advance HD III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks