BMRB Entry 34190

Title:
Proteome-wide analysis of phospho-regulated PDZ domain interactions
Deposition date:
2017-10-24
Original release date:
2018-08-31
Authors:
Chi, N.
Citation:

Citation: Sundell, Gustav; Arnold, Roland; Ali, Muhammad; Naksukpaiboon, Piangfan; Orts, Julien; Guntert, Peter; Chi, Celestine; Ivarsson, Ylva. "Proteome-wide analysis of phospho-regulated PDZ domain interactions"  Mol. Syst. Biol. 14, e8129-e8129 (2018).
PubMed: 30126976

Assembly members:

Assembly members:
entity_1, polymer, 116 residues, 12217.792 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts69
15N chemical shifts107
1H chemical shifts648

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 116 residues - 12217.792 Da.

1   GLYALAMETGLYALAPROALAARGILEGLU
2   GLUGLUGLULEUTHRLEUTHRILELEUARG
3   GLNTHRGLYGLYLEUGLYILESERILEALA
4   GLYGLYLYSGLYSERTHRPROTYRLYSGLY
5   ASPASPGLUGLYILEPHEILESERARGVAL
6   SERGLUGLUGLYPROALAALAARGALAGLY
7   VALARGVALGLYASPLYSLEULEUGLUVAL
8   ASNGLYVALALALEUGLNGLYALAGLUHIS
9   HISGLUALAVALGLUALALEUARGGLYALA
10   GLYTHRALAVALGLNMETARGVALTRPARG
11   GLUARGMETVALGLUPROGLUASNALAVAL
12   THRILETHRPROLEUARG

Samples:

sample_1: Scribble PDZ1, [U-100% 13C], 100%

sample_conditions_1: pH: 6.5; pressure: 1 Pa; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-1H TOCSYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-1H NOESYsample_1isotropicsample_conditions_1
3J HNHAsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

CcpNMR, CCPN - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks