BMRB Entry 34161

Name: Solution structure of the complex of TRPV5(655-725) with a Calmodulin E32Q/E68Q double mutant
Published: 2018-04-20

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PDB ID: 5oeo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34161
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the complex of TRPV5(655-725) with a Calmodulin E32Q/E68Q double mutant

Deposition date:

2017-07-09

Original release date:

2018-04-20

Authors:

Vuister, G.; Bokhovchuk, F.; Bate, N.; Kovalevskaya, N.; Goult, B.; Spronk, C.

Citation:

Citation: Bokhovchuk, F.; Bate, N.; Kovalevskaya, N.; Goult, B.; Spronk, C.; Vuister, G.. "The Structural Basis of Calcium Dependent Inactivation of the Transient Receptor Potential Vanilloid 5 Channel." Biochemistry 57, 2623-2635 (2018).
PubMed: 29584409

Assembly members:

Assembly members:
entity_1, polymer, 150 residues, 16937.652 Da.
entity_2, polymer, 73 residues, 7722.194 Da.
entity_CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SMADQLTEEQIAEFKEAFSL FDKDGDGTITTKQLGTVMRS LGQNPTEAELQDMINEVDAD GNGTIDFPQFLTMMARKMKD TDSEEEIREAFRVFDKDGNG YISAAELRHVMTNLGEKLTD EEVDEMIREADIDGDGQVNY EEFVQMMTAK
entity_2: GADKEDDQEHPSEKQPSGAE SGTLARASLALPTSSLSRTA SQSSSHRGWEILRQNTLGHL NLGLNLSEGDGEE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	482
15N chemical shifts	181
1H chemical shifts	1103

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	2
3	entity_3, 1	3
4	entity_3, 2	3

Entities:

Entity 1, entity_1 150 residues - 16937.652 Da.

1	SER	MET	ALA	ASP	GLN	LEU	THR	GLU	GLU	GLN
2	ILE	ALA	GLU	PHE	LYS	GLU	ALA	PHE	SER	LEU
3	PHE	ASP	LYS	ASP	GLY	ASP	GLY	THR	ILE	THR
4	THR	LYS	GLN	LEU	GLY	THR	VAL	MET	ARG	SER
5	LEU	GLY	GLN	ASN	PRO	THR	GLU	ALA	GLU	LEU
6	GLN	ASP	MET	ILE	ASN	GLU	VAL	ASP	ALA	ASP
7	GLY	ASN	GLY	THR	ILE	ASP	PHE	PRO	GLN	PHE
8	LEU	THR	MET	MET	ALA	ARG	LYS	MET	LYS	ASP
9	THR	ASP	SER	GLU	GLU	GLU	ILE	ARG	GLU	ALA
10	PHE	ARG	VAL	PHE	ASP	LYS	ASP	GLY	ASN	GLY
11	TYR	ILE	SER	ALA	ALA	GLU	LEU	ARG	HIS	VAL
12	MET	THR	ASN	LEU	GLY	GLU	LYS	LEU	THR	ASP
13	GLU	GLU	VAL	ASP	GLU	MET	ILE	ARG	GLU	ALA
14	ASP	ILE	ASP	GLY	ASP	GLY	GLN	VAL	ASN	TYR
15	GLU	GLU	PHE	VAL	GLN	MET	MET	THR	ALA	LYS

Entity 2, entity_2 73 residues - 7722.194 Da.

1

GLY

ALA

ASP

LYS

GLU

ASP

GLN

GLU

HIS

2

PRO

SER

GLU

LYS

GLN

PRO

SER

GLY

ALA

GLU

3

SER

GLY

THR

LEU

ALA

ARG

ALA

SER

LEU

ALA

4

LEU

PRO

THR

SER

LEU

SER

ARG

THR

ALA

5

SER

GLN

SER

HIS

ARG

GLY

TRP

GLU

6

ILE

LEU

ARG

GLN

ASN

THR

LEU

GLY

HIS

LEU

7

ASN

LEU

GLY

LEU

ASN

LEU

SER

GLU

GLY

ASP

8

GLY

GLU

Entity 3, entity_3, 1 - Ca - 40.078 Da.

1

CA

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 34161

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	SER	MET	ALA	ASP	GLN	LEU	THR	GLU	GLU	GLN
2	ILE	ALA	GLU	PHE	LYS	GLU	ALA	PHE	SER	LEU
3	PHE	ASP	LYS	ASP	GLY	ASP	GLY	THR	ILE	THR
4	THR	LYS	GLN	LEU	GLY	THR	VAL	MET	ARG	SER
5	LEU	GLY	GLN	ASN	PRO	THR	GLU	ALA	GLU	LEU
6	GLN	ASP	MET	ILE	ASN	GLU	VAL	ASP	ALA	ASP
7	GLY	ASN	GLY	THR	ILE	ASP	PHE	PRO	GLN	PHE
8	LEU	THR	MET	MET	ALA	ARG	LYS	MET	LYS	ASP
9	THR	ASP	SER	GLU	GLU	GLU	ILE	ARG	GLU	ALA
10	PHE	ARG	VAL	PHE	ASP	LYS	ASP	GLY	ASN	GLY
11	TYR	ILE	SER	ALA	ALA	GLU	LEU	ARG	HIS	VAL
12	MET	THR	ASN	LEU	GLY	GLU	LYS	LEU	THR	ASP
13	GLU	GLU	VAL	ASP	GLU	MET	ILE	ARG	GLU	ALA
14	ASP	ILE	ASP	GLY	ASP	GLY	GLN	VAL	ASN	TYR
15	GLU	GLU	PHE	VAL	GLN	MET	MET	THR	ALA	LYS

1	SER	MET	ALA	ASP	GLN	LEU	THR	GLU	GLU	GLN
2	ILE	ALA	GLU	PHE	LYS	GLU	ALA	PHE	SER	LEU
3	PHE	ASP	LYS	ASP	GLY	ASP	GLY	THR	ILE	THR
4	THR	LYS	GLN	LEU	GLY	THR	VAL	MET	ARG	SER
5	LEU	GLY	GLN	ASN	PRO	THR	GLU	ALA	GLU	LEU
6	GLN	ASP	MET	ILE	ASN	GLU	VAL	ASP	ALA	ASP
7	GLY	ASN	GLY	THR	ILE	ASP	PHE	PRO	GLN	PHE
8	LEU	THR	MET	MET	ALA	ARG	LYS	MET	LYS	ASP
9	THR	ASP	SER	GLU	GLU	GLU	ILE	ARG	GLU	ALA
10	PHE	ARG	VAL	PHE	ASP	LYS	ASP	GLY	ASN	GLY
11	TYR	ILE	SER	ALA	ALA	GLU	LEU	ARG	HIS	VAL
12	MET	THR	ASN	LEU	GLY	GLU	LYS	LEU	THR	ASP
13	GLU	GLU	VAL	ASP	GLU	MET	ILE	ARG	GLU	ALA
14	ASP	ILE	ASP	GLY	ASP	GLY	GLN	VAL	ASN	TYR
15	GLU	GLU	PHE	VAL	GLN	MET	MET	THR	ALA	LYS

1	SER	MET	ALA	ASP	GLN	LEU	THR	GLU	GLU	GLN
2	ILE	ALA	GLU	PHE	LYS	GLU	ALA	PHE	SER	LEU
3	PHE	ASP	LYS	ASP	GLY	ASP	GLY	THR	ILE	THR
4	THR	LYS	GLN	LEU	GLY	THR	VAL	MET	ARG	SER
5	LEU	GLY	GLN	ASN	PRO	THR	GLU	ALA	GLU	LEU
6	GLN	ASP	MET	ILE	ASN	GLU	VAL	ASP	ALA	ASP
7	GLY	ASN	GLY	THR	ILE	ASP	PHE	PRO	GLN	PHE
8	LEU	THR	MET	MET	ALA	ARG	LYS	MET	LYS	ASP
9	THR	ASP	SER	GLU	GLU	GLU	ILE	ARG	GLU	ALA
10	PHE	ARG	VAL	PHE	ASP	LYS	ASP	GLY	ASN	GLY
11	TYR	ILE	SER	ALA	ALA	GLU	LEU	ARG	HIS	VAL
12	MET	THR	ASN	LEU	GLY	GLU	LYS	LEU	THR	ASP
13	GLU	GLU	VAL	ASP	GLU	MET	ILE	ARG	GLU	ALA
14	ASP	ILE	ASP	GLY	ASP	GLY	GLN	VAL	ASN	TYR
15	GLU	GLU	PHE	VAL	GLN	MET	MET	THR	ALA	LYS