BMRB Entry 34103

Title:
Structure of TRBP dsRBD 1 and 2 in complex with a 19 bp siRNA (Complex B)
Deposition date:
2017-02-23
Original release date:
2018-02-19
Authors:
Masliah, G.; Maris, C.; Allain, H.
Citation:

Citation: Masliah, G.; Maris, C.; Koenig, L.; Yulikov, M.; Aeschimann, F.; Malinowska, A.; Mabille, J.; Weiler, J.; Holla, A.; Meisner-Kober, N.; Meisner-Kober, N.; Schuler, B.; Jeschke, G.; Allain, H.. "Structural basis of siRNA recognition by TRBP double-stranded RNA binding domains"  Embo J. 37, e97089-e97089 (2018).
PubMed: 29449323

Assembly members:

Assembly members:
entity_1, polymer, 215 residues, 23012.332 Da.
entity_2, polymer, 21 residues, 6534.828 Da.
entity_3, polymer, 21 residues, 6747.062 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts484
15N chemical shifts182
1H chemical shifts1076

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22
3entity_33

Entities:

Entity 1, entity_1 215 residues - 23012.332 Da.

1   GLYGLYSERLEUPROSERILEGLUGLNMET
2   LEUALAALAASNPROGLYLYSTHRPROILE
3   SERLEULEUGLNGLUTYRGLYTHRARGILE
4   GLYLYSTHRPROVALTYRASPLEULEULYS
5   ALAGLUGLYGLNALAHISGLNPROASNPHE
6   THRPHEARGVALTHRVALGLYASPTHRSER
7   CYSTHRGLYGLNGLYPROSERLYSLYSALA
8   ALALYSHISLYSALAALAGLUVALALALEU
9   LYSHISLEULYSGLYGLYSERMETLEUGLU
10   PROALALEUGLUASPSERSERSERPHESER
11   PROLEUASPSERSERLEUPROGLUASPILE
12   PROVALPHETHRALAALAALAALAALATHR
13   PROVALPROSERVALVALLEUTHRARGSER
14   PROPROMETGLULEUGLNPROPROVALSER
15   PROGLNGLNSERGLUCYSASNPROVALGLY
16   ALALEUGLNGLULEUVALVALGLNLYSGLY
17   TRPARGLEUPROGLUTYRTHRVALTHRGLN
18   GLUSERGLYPROALAHISARGLYSGLUPHE
19   THRMETTHRCYSARGVALGLUARGPHEILE
20   GLUILEGLYSERGLYTHRSERLYSLYSLEU
21   ALALYSARGASNALAALAALALYSMETLEU
22   LEUARGVALHISTHR

Entity 2, entity_2 21 residues - 6534.828 Da.

1   UUAAUUAUCU
2   AUUCCGUACU
3   U

Entity 3, entity_3 21 residues - 6747.062 Da.

1   GUACGGAAUA
2   GAUAAUUAAU
3   U

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