BMRB Entry 34043

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PDB ID: 5lw8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34043
MolProbity Validation Chart

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Title:
Salt-inducible protein splicing by inteins from extremely halophilic archaea and application to scarless segmental labeling of TonB protein
Deposition date:
2016-09-15
Original release date:
2016-10-14
Authors:
Iwai, H.; Ciragan, A.; Tascon, I.; Aranko, S.
Citation:

Citation: Ciragan, Annika; Aranko, A Sesilja; Tascon, Igor; Iwai, Hideo. "Salt-inducible Protein Splicing in cis and trans by Inteins from Extremely Halophilic Archaea as a Novel Protein-Engineering Tool"  J. Mol. Biol. 428, 4573-4588 (2016).
PubMed: 27720988

Assembly members:

Assembly members:
Protein TonB, polymer, 92 residues, 10151.667 Da.

Natural source:

Natural source:   Common Name: e-proteobacteria   Taxonomy ID: 85962   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Helicobacter Helicobacter pylori

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Protein TonB: SNEFLMKIQTAISSKNRYPK MAQIRGIEGEVLVSFTINAD GSVTDIKVVKSNTTDILNHA ALEAIKSAAHLFPKPEETVH LKIPIAYSLKED

Data sets:
Data typeCount
13C chemical shifts384
15N chemical shifts92
1H chemical shifts656

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 92 residues - 10151.667 Da.

1   SERASNGLUPHELEUMETLYSILEGLNTHR
2   ALAILESERSERLYSASNARGTYRPROLYS
3   METALAGLNILEARGGLYILEGLUGLYGLU
4   VALLEUVALSERPHETHRILEASNALAASP
5   GLYSERVALTHRASPILELYSVALVALLYS
6   SERASNTHRTHRASPILELEUASNHISALA
7   ALALEUGLUALAILELYSSERALAALAHIS
8   LEUPHEPROLYSPROGLUGLUTHRVALHIS
9   LEULYSILEPROILEALATYRSERLEULYS
10   GLUASP

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SPARKY: Backbone or all simulated peaks