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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34013
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Uzarska, M.; Nasta, V.; Weiler, B.; Spantgar, F.; Ciofi-Baffoni, S.; Saviello, M.; Gonnelli, L.; M hlenhoff, U.; Banci, L.; Lill, R.. "Mitochondrial Bol1 and Bol3 function as assembly factors for specific iron-sulfur proteins" Elife 5, e16673-e16673 (2016).
PubMed: 27532772
Assembly members:
BolA-like protein 1, polymer, 123 residues, 12875.399 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET151
Entity Sequences (FASTA):
BolA-like protein 1: GIDPFTQGSAGSGAIGPVEA
AIRTKLEEALSPEVLELRNE
SGGHAVPPGSETHFRVAVVS
SRFEGLSPLQRHRLVHAALA
EELGGPVHALAIQARTPAQW
RENSQLDTSPPCLGGNKKTL
GTP
Data type | Count |
13C chemical shifts | 465 |
15N chemical shifts | 126 |
1H chemical shifts | 778 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | entity_1 | 1 |
Entity 1, entity_1 123 residues - 12875.399 Da.
1 | GLY | ILE | ASP | PRO | PHE | THR | GLN | GLY | SER | ALA | ||||
2 | GLY | SER | GLY | ALA | ILE | GLY | PRO | VAL | GLU | ALA | ||||
3 | ALA | ILE | ARG | THR | LYS | LEU | GLU | GLU | ALA | LEU | ||||
4 | SER | PRO | GLU | VAL | LEU | GLU | LEU | ARG | ASN | GLU | ||||
5 | SER | GLY | GLY | HIS | ALA | VAL | PRO | PRO | GLY | SER | ||||
6 | GLU | THR | HIS | PHE | ARG | VAL | ALA | VAL | VAL | SER | ||||
7 | SER | ARG | PHE | GLU | GLY | LEU | SER | PRO | LEU | GLN | ||||
8 | ARG | HIS | ARG | LEU | VAL | HIS | ALA | ALA | LEU | ALA | ||||
9 | GLU | GLU | LEU | GLY | GLY | PRO | VAL | HIS | ALA | LEU | ||||
10 | ALA | ILE | GLN | ALA | ARG | THR | PRO | ALA | GLN | TRP | ||||
11 | ARG | GLU | ASN | SER | GLN | LEU | ASP | THR | SER | PRO | ||||
12 | PRO | CYS | LEU | GLY | GLY | ASN | LYS | LYS | THR | LEU | ||||
13 | GLY | THR | PRO |
sample_1: DTT 5 mM; mitochondrial BOLA1 protein, [U-100% 15N], 1 mM; potassium phosphate 50 mM; H2O 90%; D2O 10%
sample_2: DTT 5 mM; mitochondrial BOLA1 protein, [U-100% 13C; U-100% 15N], 1 mM; potassium phosphate 50 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K
sample_conditions_2: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | anisotropic | sample_conditions_1 |
2D 1H-13C HSQC aliphatic | sample_2 | anisotropic | sample_conditions_2 |
3D HNCO | sample_2 | anisotropic | sample_conditions_2 |
3D HNCA | sample_2 | anisotropic | sample_conditions_2 |
3D HN(CO)CA | sample_2 | anisotropic | sample_conditions_2 |
3D HBHA(CBCACO)NH | sample_2 | anisotropic | sample_conditions_2 |
3D HN(CA)CO | sample_2 | anisotropic | sample_conditions_2 |
3D CBCA(CO)NH | sample_2 | anisotropic | sample_conditions_2 |
3D HNCACB | sample_2 | anisotropic | sample_conditions_2 |
3D HNCACB | sample_2 | anisotropic | sample_conditions_2 |
3D 1H-13C NOESY | sample_2 | anisotropic | sample_conditions_2 |
3D HCCH-TOCSY | sample_2 | anisotropic | sample_conditions_2 |
2D 1H-1H TOCSY | sample_1 | anisotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | anisotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | anisotropic | sample_conditions_1 |
AMBER, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, and Kollman - refinement
CARA, Keller and Wuthrich - peak picking
CARA v2, Keller and Wuthrich - chemical shift assignment
TALOS+, Cornilescu, Delaglio and Bax - data analysis
TOPSPIN, Bruker Biospin - collection, processing
UNIO, Serrano, Pedrini, Mohanty, Geralt, Herrmann and Wuthrich, - peak picking, structure calculation
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks