BMRB Entry 34008

Title:
NMR structure of the sea anemone peptide tau-AnmTx Ueq 12-1 with an uncommon fold
Deposition date:
2016-06-14
Original release date:
2017-05-04
Authors:
Mineev, K.; Arseniev, A.; Andreev, A.; Kozlov, S.; Logashina, Yu.
Citation:

Citation: Logashina, Yulia; Solstad, Runar Gjerp; Mineev, Konstantin; Korolkova, Yuliya; Mosharova, Irina; Dyachenko, Igor; Palikov, Victor; Palikova, Yulia; Murashev, Arkadii; Arseniev, Alexander; Kozlov, Sergey; Stensvag, Klara; Haug, Tor; Andreev, Yaroslav. "New Disulfide-Stabilized Fold Provides Sea Anemone Peptide to Exhibit Both Antimicrobial and TRPA1 Potentiating Properties"  Toxins (Basel) 9, 154-154 (2017).
PubMed: 28468269

Assembly members:

Assembly members:
tau-AnmTx Ueq 12-1, polymer, 45 residues, 4808.258 Da.

Natural source:

Natural source:   Common Name: sea anemones   Taxonomy ID: 417072   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Urticina eques

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
tau-AnmTx Ueq 12-1: CYPGQPGCGHCSRPNYCEGA RCESGFHDCGSDHWCDASGD RCCCA

Data sets:
Data typeCount
13C chemical shifts99
15N chemical shifts45
1H chemical shifts233

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 45 residues - 4808.258 Da.

1   CYSTYRPROGLYGLNPROGLYCYSGLYHIS
2   CYSSERARGPROASNTYRCYSGLUGLYALA
3   ARGCYSGLUSERGLYPHEHISASPCYSGLY
4   SERASPHISTRPCYSASPALASERGLYASP
5   ARGCYSCYSCYSALA

Samples:

sample_1: sodium azide 1 mM; tau-AnmTx Ueq 12-1 0.5 ± 0.1 mM; H2O 95%; D2O 5%

sample_2: sodium azide 1 mM; tau-AnmTx Ueq 12-1 0.5 ± 0.1 mM; D2O 100%

sample_conditions_1: ionic strength: 10 mM; pH: 3.2; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D DQF-COSYsample_2isotropicsample_conditions_1

Software:

CARA v1.9.4, Keller and Wuthrich - chemical shift assignment

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure calculation

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks