BMRB Entry 31235

Name: NMR structure of conotoxin k-SrXIA
Published: 2025-05-08

Click here to enlarge.

PDB ID: 9nvh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31235
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

NMR structure of conotoxin k-SrXIA

Deposition date:

2025-03-20

Original release date:

2025-05-08

Authors:

Melchor-Meneses, C.; del Rio-Portilla, F.

Citation:

Citation: Escobar-Perez, L.; Melchor-Meneses, C.; del Rio-Portilla, F.. "Block of Kv1 potassium channels and NMR structure of the recombinant conotoxin k-SrXIA" .

Assembly members:

Assembly members:
entity_1, polymer, 35 residues, 3865.345 Da.

Natural source:

Natural source: Common Name: Alphabet cone Taxonomy ID: 192919 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Conus spurius

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGSCRTEGMSCEENQQCCWR SCCRGECEAPCRFGP

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
1H chemical shifts	188

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 35 residues - 3865.345 Da.

1

GLY

SER

CYS

ARG

THR

GLU

GLY

MET

SER

2

CYS

GLU

ASN

GLN

CYS

TRP

ARG

3

SER

CYS

ARG

GLY

GLU

CYS

GLU

ALA

PRO

4

CYS

ARG

PHE

GLY

PRO

Samples:

sample_1: Conotoxin k-SrXIA 1 mM

sample_conditions_1: ionic strength: 1 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

Amber, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, and Kollman - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker AVANCE III HD 700 MHz