BMRB Entry 31180

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31180
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution NMR structure of the fungal loosenin PcaLOO12 from Phanerochaete carnosa
Deposition date:
2024-06-26
Original release date:
2024-07-05
Authors:
Buchko, G.; Master, E.
Citation:

Citation: Buchko, G.; Master, E.. "Bio-SANS investigation of fungal loosenins reveal substrate dependent impacts of protein action on inter-fibril distance and packing order of cellulosic substrates"  .

Assembly members:

Assembly members:
entity_1, polymer, 105 residues, 10779.799 Da.

Natural source:

Natural source:   Common Name: Phanerochaete carnosa (strain HHB-10118-sp)   Taxonomy ID: 650164   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Phanerochaete carnosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHAPAEKRSTTREGRGTWYD TGLGACGWNNVNSDTVIALS PSVYSGGSHCGQTVTVTNVV TGAKATGTVADECPGCGPND IDMTPGLFQQLGSLDEGVLT VSWTL

Data sets:
Data typeCount
13C chemical shifts404
15N chemical shifts107
1H chemical shifts629

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 105 residues - 10779.799 Da.

1   GLYHISALAPROALAGLULYSARGSERTHR
2   THRARGGLUGLYARGGLYTHRTRPTYRASP
3   THRGLYLEUGLYALACYSGLYTRPASNASN
4   VALASNSERASPTHRVALILEALALEUSER
5   PROSERVALTYRSERGLYGLYSERHISCYS
6   GLYGLNTHRVALTHRVALTHRASNVALVAL
7   THRGLYALALYSALATHRGLYTHRVALALA
8   ASPGLUCYSPROGLYCYSGLYPROASNASP
9   ILEASPMETTHRPROGLYLEUPHEGLNGLN
10   LEUGLYSERLEUASPGLUGLYVALLEUTHR
11   VALSERTRPTHRLEU

Samples:

sample_1: PcaLOOL12, [U-99% 13C; U-99% 15N], 0.8 mM; sodium chloride 100 mM; TRIS 20 mM

sample_2: PcaLOOL12, [U-10% 13C; U-99% 15N], 0.8 mM; sodium chloride 100 mM; TRIS 20 mM

sample_3: PcaLOOL12, [U-99% 15N], 0.4 mM; sodium chloride 100 mM; TRIS 20 mM

sample_conditions_1: ionic strength: 0.12 M; pH: 7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
Deuterium exchangesample_3isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1

Software:

Poky, Manthey, Tonelli, Clos II, Rahimi, Markley and Lee - data analysis

CYANA v1.2, Guntert, Mumenthaler and Wuthrich - structure calculation

CNSSOLVE v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

TALOS+, Shen, Delaglio, Cornilescu and Bax - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PSVS v1.5, Bhattacharya and Montelione - data analysis

NMR spectrometers:

  • Varian VNMRS 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks