BMRB Entry 31098

Title:
Solution structure of the zinc finger repeat domain of BCL11A (ZnF456)
Deposition date:
2023-07-17
Original release date:
2024-07-17
Authors:
Viennet, T.; Yin, M.; Orkin, S.; Arthanari, H.
Citation:

Citation: Viennet, T.; Yin, M.; Jayaraj, A.; Kim, W.; Sun, Z.-Y.J.; Seo, H.-S.; Dhe-Paganon, S.; Orkin, S.; Arthanari, H.. "Structural and dynamic investigation of DNA-binding by BCL11A reveals a specific role for ZnF6"  .

Assembly members:

Assembly members:
entity_1, polymer, 100 residues, 11703.447 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Data sets:
Data typeCount
13C chemical shifts311
15N chemical shifts97
1H chemical shifts551

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22
3unit_32
4unit_42

Entities:

Entity 1, unit_1 100 residues - 11703.447 Da.

1   SERGLUGLYARGARGSERASPTHRCYSGLU
2   TYRCYSGLYLYSVALPHELYSASNCYSSER
3   ASNLEUTHRVALHISARGARGSERHISTHR
4   GLYGLUARGPROTYRLYSCYSGLULEUCYS
5   ASNTYRALACYSALAGLNSERSERLYSLEU
6   THRARGHISMETLYSTHRHISGLYGLNVAL
7   GLYLYSASPVALTYRLYSCYSGLUILECYS
8   LYSMETPROPHESERVALTYRSERTHRLEU
9   GLULYSHISMETLYSLYSTRPHISSERASP
10   ARGVALLEUASNASNASPILELYSTHRGLU

Entity 2, unit_2 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: sodium chloride 137 mM; potassium chloride 2.7 mM; sodium phosphate 10 mM; potassium phosphate 1.8 mM; dithiothreitol 1 mM

sample_conditions_1: ionic strength: 163 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

TopSpin v3.2, Bruker Biospin - collection

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CcpNmr Analysis v2.4.2, CCPN - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Amber, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, and Kollman - refinement

NMR spectrometers:

  • Bruker AVANCE III HD 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks