BMRB Entry 31086

Title:
Antimicrobial lasso peptide achromonodin-1
Deposition date:
2023-05-16
Original release date:
2023-10-07
Authors:
Carson, D.; Cheung-Lee, W.; So, L.; Link, A.
Citation:

Citation: Carson, Drew; Zhang, Yi; So, Larry; Cheung-Lee, Wai Ling; Cartagena, Alexis Jaramillo; Darst, Seth; Link, A James. "Discovery, Characterization, and Bioactivity of the Achromonodins: Lasso Peptides Encoded by Achromobacter"  J. Nat. Prod. 86, 2448-2456 (2023).
PubMed: 37870195

Assembly members:

Assembly members:
entity_1, polymer, 30 residues, 3220.588 Da.

Natural source:

Natural source:   Common Name: Achromobacter xylosoxidans   Taxonomy ID: 85698   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Achromobacter xylosoxidans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGGGPTPEYFLMPIDPAWLQ ANLPNTGKYN

Data sets:
Data typeCount
1H chemical shifts196

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 30 residues - 3220.588 Da.

1   GLYGLYGLYGLYPROTHRPROGLUTYRPHE
2   LEUMETPROILEASPPROALATRPLEUGLN
3   ALAASNLEUPROASNTHRGLYLYSTYRASN

Samples:

sample_1: Achromonodin-1 10 mg/mL

sample_conditions_1: ionic strength: 0 mM; pH: 7; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

MestreLab (Mnova / MestReNova / MestReC) v14.2.0-26256, MestreLab Research - chemical shift assignment, peak picking

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

NMR spectrometers:

  • Bruker AVANCE III 800 MHz