BMRB Entry 31073
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31073
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Chandrahas, A.. "Reelin C-Terminal Region" .
Assembly members:
entity_1, polymer, 32 residues, 4225.879 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: STRKQNYMMNFSRQHGLRHF
YNRRRRSLRRYP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 92 |
| 15N chemical shifts | 33 |
| 1H chemical shifts | 216 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 32 residues - 4225.879 Da.
| 1 | SER | THR | ARG | LYS | GLN | ASN | TYR | MET | MET | ASN | ||||
| 2 | PHE | SER | ARG | GLN | HIS | GLY | LEU | ARG | HIS | PHE | ||||
| 3 | TYR | ASN | ARG | ARG | ARG | ARG | SER | LEU | ARG | ARG | ||||
| 4 | TYR | PRO |
Samples:
sample_1: Reelin CTR, [U-98% 15N], 3.3 mM; THF (CF3-CD2-OH) 50 % v/v; DSS (4,4-dimethyl-4-silapentane-1-sulfonic acid) 0.25 mM
sample_conditions_1: pH: 7.4; temperature: 298.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS v1.2.1, Brunger A. T. et.al. - refinement, structure calculation
ARIA v2.3.2, Linge, O'Donoghue and Nilges - structure calculation
PSVS, Bhattacharya and Montelione - data analysis
CcpNmr Analysis v2.4.2, CCPN - data analysis
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
PyMOL v2.5.4, Schrodinger and DeLano - data analysis
NMR spectrometers:
- Agilent Direct Drive 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks