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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31065
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Ye, Y.; Tian, F.; Tyndall, E.; Bui, V.; Bewley, M.; Wang, G.; Hong, X.; Shen, Y.; Flanagan, J.; Wang, H.. "Translating Membrane Geometry into Protein Function: Multifaceted Membrane Interactions of Human Atg3 Promote LC3-Phosphatidylethanolamine Conjugation during Autophagy" .
Assembly members:
entity_1, polymer, 189 residues, 22091.420 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Data type | Count |
13C chemical shifts | 804 |
15N chemical shifts | 165 |
1H chemical shifts | 1123 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | unit_1 | 1 |
Entity 1, unit_1 189 residues - 22091.420 Da.
1 | GLY | SER | LYS | PHE | LYS | GLU | THR | GLY | VAL | ILE | ||||
2 | THR | PRO | GLU | GLU | PHE | VAL | ALA | ALA | GLY | ASP | ||||
3 | HIS | LEU | VAL | HIS | HIS | CYS | PRO | THR | TRP | GLN | ||||
4 | TRP | ALA | THR | GLY | GLU | GLU | LEU | LYS | VAL | LYS | ||||
5 | ALA | TYR | LEU | PRO | THR | GLY | LYS | GLN | PHE | LEU | ||||
6 | VAL | THR | LYS | ASN | VAL | PRO | CYS | TYR | LYS | ARG | ||||
7 | CYS | LYS | GLN | MET | GLU | GLU | ASP | ALA | ILE | LEU | ||||
8 | GLN | THR | ARG | THR | TYR | ASP | LEU | TYR | ILE | THR | ||||
9 | TYR | ASP | LYS | TYR | TYR | GLN | THR | PRO | ARG | LEU | ||||
10 | TRP | LEU | PHE | GLY | TYR | ASP | GLU | GLN | ARG | GLN | ||||
11 | PRO | LEU | THR | VAL | GLU | HIS | MET | TYR | GLU | ASP | ||||
12 | ILE | SER | GLN | ASP | HIS | VAL | LYS | LYS | THR | VAL | ||||
13 | THR | ILE | GLU | ASN | HIS | PRO | HIS | LEU | PRO | PRO | ||||
14 | PRO | PRO | MET | CYS | SER | VAL | HIS | PRO | CYS | ARG | ||||
15 | HIS | ALA | GLU | VAL | MET | LYS | LYS | ILE | ILE | GLU | ||||
16 | THR | VAL | ALA | GLU | GLY | GLY | GLY | GLU | LEU | GLY | ||||
17 | VAL | HIS | MET | TYR | LEU | LEU | ILE | PHE | LEU | LYS | ||||
18 | PHE | VAL | GLN | ALA | VAL | ILE | PRO | THR | ILE | GLU | ||||
19 | TYR | ASP | TYR | THR | ARG | HIS | PHE | THR | MET |
sample_1: NaCl 150 mM; HEPES 50 mM; TCEP 2 mM; human Atg3, [U-13C; U-15N], 0.5 mM
sample_2: NaCl 150 mM; HEPES 50 mM; TCEP 2 mM; human Atg3, [U-13C; U-15N], 0.5 mM; Pf1 phage 6.5 mg/mL
sample_3: NaCl 150 mM; HEPES 50 mM; TCEP 2 mM; human Atg3, [U-13C; U-15N], 0.5 mM; positive gel 7%
sample_4: NaCl 150 mM; HEPES 50 mM; TCEP 2 mM; human Atg3, [U-13C; U-15N], 0.5 mM; negative gel 5%
sample_5: NaCl 150 mM; HEPES 50 mM; TCEP 2 mM; human Atg3, [U-13C; U-15N], 0.5 mM; neutral gel 5%
sample_6: NaCl 150 mM; HEPES 50 mM; TCEP 2 mM; human Atg3, [U-13C; U-15N], 0.5 mM
sample_7: NaCl 150 mM; HEPES 50 mM; TCEP 2 mM; human Atg3, [U-13C; U-15N], 0.5 mM
sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 Pa; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_6 | isotropic | sample_conditions_1 |
3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_6 | isotropic | sample_conditions_1 |
2D ARTSY | sample_7 | anisotropic | sample_conditions_1 |
2D ARTSY | sample_5 | anisotropic | sample_conditions_1 |
2D ARTSY | sample_4 | anisotropic | sample_conditions_1 |
2D ARTSY | sample_3 | anisotropic | sample_conditions_1 |
2D ARTSY | sample_2 | anisotropic | sample_conditions_1 |
HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
2D Hb(CbCgCd)Hd | sample_6 | isotropic | sample_conditions_1 |
2D Hb(CbCgCdCe)He | sample_6 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aromatic | sample_6 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aromatic | sample_6 | isotropic | sample_conditions_1 |
CNX, NIH - refinement
NMRViewJ, One Moon Scientific - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
NMRView, Johnson, One Moon Scientific - peak picking
TopSpin, Bruker Biospin - collection
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks