BMRB Entry 31026

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31026
MolProbity Validation Chart

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Title:
Intramembrane recognition between transmembrane domains of IL-7R and common gamma chain
Deposition date:
2022-06-17
Original release date:
2023-06-27
Authors:
Cai, T.; Lenoir Capello, R.; Chou, J.
Citation:

Citation: Cai, T.; Lenoir Capello, R.; Pi, X.; Wu, H.; Chou, J.. "Structural basis of gamma-chain family receptor sharing at the membrane level"  .
PubMed: 37205582

Assembly members:

Assembly members:
entity_1, polymer, 34 residues, 3864.547 Da.
entity_2, polymer, 31 residues, 3452.199 Da.

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: EENPSLFALEAVLIPVGTVG LIITLIFVYFWLER
entity_2: GGWDPVLPSVTILSLFSVFL LVILAHVLWKK

Data sets:
Data typeCount
13C chemical shifts153
15N chemical shifts50
1H chemical shifts345

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22

Entities:

Entity 1, unit_1 34 residues - 3864.547 Da.

1   GLUGLUASNPROSERLEUPHEALALEUGLU
2   ALAVALLEUILEPROVALGLYTHRVALGLY
3   LEUILEILETHRLEUILEPHEVALTYRPHE
4   TRPLEUGLUARG

Entity 2, unit_2 31 residues - 3452.199 Da.

1   GLYGLYTRPASPPROVALLEUPROSERVAL
2   THRILELEUSERLEUPHESERVALPHELEU
3   LEUVALILELEUALAHISVALLEUTRPLYS
4   LYS

Samples:

sample_1: Transmembrane domain of the common gamma-chain receptor, [U-15N; U-13C; U-85% 2H], 0.4 ± 0.02 mM; DMPC 40 ± 0.2 mM; DH6PC 100 ± 0.2 mM

sample_2: DMPC 40 ± 0.2 mM; DH6PC 100 ± 0.2 mM; Transmembrane domain of the Interleukin-7 receptor alpha subunit, [U-15N; U-13C; U-85% 2H], 0.4 ± 0.02 mM

sample_3: Transmembrane domain of the common gamma-chain receptor, [U-15N; U-2H], 0.4 ± 0.02 mM; Transmembrane domain of the Interleukin-7 receptor alpha subunit, [U-13C], 0.4 ± 0.02 mM; DMPC (deuterated), deuterated, 40 ± 0.2 mM; DH6PC (deuterated), deuterated, 100 ± 0.2 mM

sample_4: Transmembrane domain of the common gamma-chain receptor, [U-13C], 0.4 ± 0.02 mM; Transmembrane domain of the Interleukin-7 receptor alpha subunit, [U-15N; U-2H], 0.4 ± 0.02 mM; DMPC (deuterated), deuterated, 40 ± 0.2 mM; DH6PC (deuterated), deuterated, 100 ± 0.2 mM

sample_5: Transmembrane domain of the common gamma-chain receptor, [U-15N; U-13C], 0.4 ± 0.02 mM; Transmembrane domain of the Interleukin-7 receptor alpha subunit, [U-15N; U-13C], 0.4 ± 0.02 mM; DMPC (deuterated), deuterated, 40 ± 0.2 mM; DH6PC (deuterated), deuterated, 100 ± 0.2 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSY-HSQCsample_1isotropicsample_conditions_1
2D 1H-15N TROSY-HSQCsample_2isotropicsample_conditions_1
2D 1H-15N TROSY-HSQCsample_3isotropicsample_conditions_1
2D 1H-15N TROSY-HSQCsample_4isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
2D 1H-13C HSQCsample_4isotropicsample_conditions_1
3D TROSY HNCAsample_1isotropicsample_conditions_1
3D TROSY HNCAsample_2isotropicsample_conditions_1
3D TROSY HN(CO)CAsample_1isotropicsample_conditions_1
3D TROSY HN(CO)CAsample_2isotropicsample_conditions_1
3D TROSY HNCOsample_1isotropicsample_conditions_1
3D TROSY HNCOsample_2isotropicsample_conditions_1
3D TROSY HNCACOsample_1isotropicsample_conditions_1
3D TROSY HNCACOsample_2isotropicsample_conditions_1
3D 1H-15N NOESY-TROSYsample_3isotropicsample_conditions_1
3D 1H-15N NOESY-TROSYsample_4isotropicsample_conditions_1
3D 1H-15N NOESY-TROSYsample_5isotropicsample_conditions_1
3D 1H-13C NOESYsample_3isotropicsample_conditions_1
3D 1H-13C NOESYsample_4isotropicsample_conditions_1
2D Methyl-Amide Intermolecular NOESYsample_3isotropicsample_conditions_1
3D 1H-13C NOESYsample_5isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment

TALOS+, Shen, Delaglio, Cornilescu, Bax - chemical shift calculation

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker AVANCE III 600 MHz
  • Bruker AVANCE NEO 700 MHz
  • Bruker AVANCE III 800 MHz
  • Bruker AVANCE III 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks