BMRB Entry 31026

Name: Intramembrane recognition between transmembrane domains of IL-7R and common gamma chain
Published: 2023-06-27

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PDB ID: 8ddc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31026
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Intramembrane recognition between transmembrane domains of IL-7R and common gamma chain

Deposition date:

2022-06-17

Original release date:

2023-06-27

Authors:

Cai, T.; Lenoir Capello, R.; Chou, J.

Citation:

Citation: Cai, T.; Lenoir Capello, R.; Pi, X.; Wu, H.; Chou, J.. "Structural basis of gamma-chain family receptor sharing at the membrane level" .
PubMed: 37205582

Assembly members:

Assembly members:
entity_1, polymer, 34 residues, 3864.547 Da.
entity_2, polymer, 31 residues, 3452.199 Da.

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: EENPSLFALEAVLIPVGTVG LIITLIFVYFWLER
entity_2: GGWDPVLPSVTILSLFSVFL LVILAHVLWKK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	153
15N chemical shifts	50
1H chemical shifts	345

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1
2	unit_2	2

Entities:

Entity 1, unit_1 34 residues - 3864.547 Da.

1

GLU

ASN

PRO

SER

LEU

PHE

ALA

LEU

GLU

2

ALA

VAL

LEU

ILE

PRO

VAL

GLY

THR

VAL

GLY

3

LEU

ILE

THR

LEU

ILE

PHE

VAL

TYR

PHE

4

TRP

LEU

GLU

ARG

Entity 2, unit_2 31 residues - 3452.199 Da.

1

GLY

TRP

ASP

PRO

VAL

LEU

PRO

SER

VAL

2

THR

ILE

LEU

SER

LEU

PHE

SER

VAL

PHE

LEU

3

LEU

VAL

ILE

LEU

ALA

HIS

VAL

LEU

TRP

LYS

4

LYS

Samples:

sample_1: Transmembrane domain of the common gamma-chain receptor, [U-15N; U-13C; U-85% 2H], 0.4 ± 0.02 mM; DMPC 40 ± 0.2 mM; DH6PC 100 ± 0.2 mM

sample_2: DMPC 40 ± 0.2 mM; DH6PC 100 ± 0.2 mM; Transmembrane domain of the Interleukin-7 receptor alpha subunit, [U-15N; U-13C; U-85% 2H], 0.4 ± 0.02 mM

sample_3: Transmembrane domain of the common gamma-chain receptor, [U-15N; U-2H], 0.4 ± 0.02 mM; Transmembrane domain of the Interleukin-7 receptor alpha subunit, [U-13C], 0.4 ± 0.02 mM; DMPC (deuterated), deuterated, 40 ± 0.2 mM; DH6PC (deuterated), deuterated, 100 ± 0.2 mM

sample_4: Transmembrane domain of the common gamma-chain receptor, [U-13C], 0.4 ± 0.02 mM; Transmembrane domain of the Interleukin-7 receptor alpha subunit, [U-15N; U-2H], 0.4 ± 0.02 mM; DMPC (deuterated), deuterated, 40 ± 0.2 mM; DH6PC (deuterated), deuterated, 100 ± 0.2 mM

sample_5: Transmembrane domain of the common gamma-chain receptor, [U-15N; U-13C], 0.4 ± 0.02 mM; Transmembrane domain of the Interleukin-7 receptor alpha subunit, [U-15N; U-13C], 0.4 ± 0.02 mM; DMPC (deuterated), deuterated, 40 ± 0.2 mM; DH6PC (deuterated), deuterated, 100 ± 0.2 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.7; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY-HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N TROSY-HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-15N TROSY-HSQC	sample_3	isotropic	sample_conditions_1
2D 1H-15N TROSY-HSQC	sample_4	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_4	isotropic	sample_conditions_1
3D TROSY HNCA	sample_1	isotropic	sample_conditions_1
3D TROSY HNCA	sample_2	isotropic	sample_conditions_1
3D TROSY HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D TROSY HN(CO)CA	sample_2	isotropic	sample_conditions_1
3D TROSY HNCO	sample_1	isotropic	sample_conditions_1
3D TROSY HNCO	sample_2	isotropic	sample_conditions_1
3D TROSY HNCACO	sample_1	isotropic	sample_conditions_1
3D TROSY HNCACO	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY-TROSY	sample_3	isotropic	sample_conditions_1
3D 1H-15N NOESY-TROSY	sample_4	isotropic	sample_conditions_1
3D 1H-15N NOESY-TROSY	sample_5	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_3	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_4	isotropic	sample_conditions_1
2D Methyl-Amide Intermolecular NOESY	sample_3	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_5	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks