BMRB Entry 30807

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PDB ID: 7kix
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30807
MolProbity Validation Chart

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Title:
The structure of anti-CRISPR AcrIE2
Deposition date:
2020-10-25
Original release date:
2021-05-21
Authors:
Pawluk, A.; Davidson, A.; Maxwell, K.
Citation:

Citation: Mejdani, M.; Pawluk, A.; Maxwell, K.; Davidson, A.. "Anti-CRISPR AcrIE2 Binds the Type I-E CRISPR-Cas Complex But Does Not Block DNA Binding"  J. Mol. Biol. 433, 166759-166759 (2021).
PubMed: 33338493

Assembly members:

Assembly members:
entity_1, polymer, 91 residues, 10319.222 Da.

Natural source:

Natural source:   Common Name: Pseudomonas phage JBD16C   Taxonomy ID: 1777051   Superkingdom: Viruses   Kingdom: not available   Genus/species: Casadabanvirus Pseudomonas phage JBD16C

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MNTYLIDPRKNNDNSGERFT VDAVDITAAAKSAAQQILGE EFEGLVYRETGESNGSGMFQ AYHHLHGTNRTETTVGYPFH VMELEHHHHHH

Data sets:
Data typeCount
13C chemical shifts309
15N chemical shifts84
1H chemical shifts504

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 91 residues - 10319.222 Da.

1   METASNTHRTYRLEUILEASPPROARGLYS
2   ASNASNASPASNSERGLYGLUARGPHETHR
3   VALASPALAVALASPILETHRALAALAALA
4   LYSSERALAALAGLNGLNILELEUGLYGLU
5   GLUPHEGLUGLYLEUVALTYRARGGLUTHR
6   GLYGLUSERASNGLYSERGLYMETPHEGLN
7   ALATYRHISHISLEUHISGLYTHRASNARG
8   THRGLUTHRTHRVALGLYTYRPROPHEHIS
9   VALMETGLULEUGLUHISHISHISHISHIS
10   HIS

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