BMRB Entry 30672

Title:
SMARCB1 nucleosome-interacting C-terminal alpha helix
Deposition date:
2019-09-16
Original release date:
2019-11-22
Authors:
Valencia, A.; Sun, Z.; Seo, H.; Mashtalir, N.; Dhe-Paganon, S.; Kadoch, C.
Citation:

Citation: Valencia, Alfredo; Collings, Clayton; Dao, Hai; St Pierre, Roodolph; Cheng, Yung-Chih; Huang, Junwei; Sun, Zhen-Yu; Seo, Hyuk-Soo; Mashtalir, Nazar; Comstock, Dawn; Bolonduro, Olubusayo; Vangos, Nicholas; Yeoh, Zoe; Dornon, Mary Kate; Hermawan, Crystal; Barrett, Lee; Dhe-Paganon, Sirano; Woolf, Clifford; Muir, Tom; Kadoch, Cigall. "Recurrent SMARCB1 Mutations Reveal a Nucleosome Acidic Patch Interaction Site That Potentiates mSWI/SNF Complex Chromatin Remodeling"  Cell 179, 1342-1356 (2019).
PubMed: 31759698

Assembly members:

Assembly members:
entity_1, polymer, 41 residues, 4748.473 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPLGSMPLLETLTDAEMEKK IRDQDRNTRRMRRLANTAPA W

Data sets:
Data typeCount
13C chemical shifts172
15N chemical shifts41
1H chemical shifts282

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 41 residues - 4748.473 Da.

1   GLYPROLEUGLYSERMETPROLEULEUGLU
2   THRLEUTHRASPALAGLUMETGLULYSLYS
3   ILEARGASPGLNASPARGASNTHRARGARG
4   METARGARGLEUALAASNTHRALAPROALA
5   TRP

Samples:

sample_1: SMARCB1CC, [U-100% 13C; U-100% 15N], 300 uM; NaCl 137 mM; potassium chloride 2.7 mM; Na2HPO4 10 mM; KH2PO4 1.8 mM

sample_2: SMARCB1CC 230 uM; NaCl 137 mM; potassium chloride 2.7 mM; Na2HPO4 10 mM; KH2PO4 1.8 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
2D TOCSYsample_2isotropicsample_conditions_1
2D NOESYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1

Software:

VNMR, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

hmsIST, Hyberts and Wagner - processing

CARA, Keller and Wuthrich - data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Agilent DD2 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks