BMRB Entry 30653

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30653
MolProbity Validation Chart

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Title:
Human CstF-64 RRM mutant - D50A
Deposition date:
2019-08-12
Original release date:
2020-08-14
Authors:
Latham, M.; Masoumzadeh, E.
Citation:

Citation: Grozdanov, Petar; Masoumzadeh, Elahe; Kalscheuer, Vera; Bienvenu, Thierry; Billuart, Pierre; Delrue, Marie-Ange; Latham, Michael; MacDonald, Clinton. "A missense mutation in the CSTF2 gene that impairs the function of the RNA recognition motif and causes defects in 3' end processing is associated with intellectual disability in humans "  Nucleic Acids Res. 48, 9804-9821 (2020).
PubMed: 32816001

Assembly members:

Assembly members:
entity_1, polymer, 110 residues, 12117.579 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAGLTVRDPAVDRSLRSVFV GNIPYEATEEQLKDIFSEVG PVVSFRLVYARETGKPKGYG FCEYQDQETALSAMRNLNGR EFSGRALRVDNAASEKNKEE LKSLGTGAPV

Data sets:
Data typeCount
13C chemical shifts223
15N chemical shifts100
1H chemical shifts100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 110 residues - 12117.579 Da.

1   METALAGLYLEUTHRVALARGASPPROALA
2   VALASPARGSERLEUARGSERVALPHEVAL
3   GLYASNILEPROTYRGLUALATHRGLUGLU
4   GLNLEULYSASPILEPHESERGLUVALGLY
5   PROVALVALSERPHEARGLEUVALTYRALA
6   ARGGLUTHRGLYLYSPROLYSGLYTYRGLY
7   PHECYSGLUTYRGLNASPGLNGLUTHRALA
8   LEUSERALAMETARGASNLEUASNGLYARG
9   GLUPHESERGLYARGALALEUARGVALASP
10   ASNALAALASERGLULYSASNLYSGLUGLU
11   LEULYSSERLEUGLYTHRGLYALAPROVAL

Samples:

sample_1: CstF-64 RNA recognition motif mutant, [U-100% 13C; U-100% 15N], 1.2 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CcpNmr Analysis v2.4.2, CCPN - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, peak picking

CS-ROSETTA, Shen, Vernon, Baker and Bax - refinement, structure calculation

NMR spectrometers:

  • Agilent DD2 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks