BMRB Entry 30652
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30652
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Grozdanov, Petar; Masoumzadeh, Elahe; Kalscheuer, Vera; Bienvenu, Thierry; Billuart, Pierre; Delrue, Marie-Ange; Latham, Michael; MacDonald, Clinton. "A missense mutation in the CSTF2 gene that impairs the function of the RNA recognition motif and causes defects in 3' end processing is associated with intellectual disability in humans
" Nucleic Acids Res. 48, 9804-9821 (2020).
PubMed: 32816001
Assembly members:
entity_1, polymer, 108 residues, 11965.345 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22
Entity Sequences (FASTA):
entity_1: MAGLTVRDPAVDRSLRSVFV
GNIPYEATEEQLKDIFSEVG
PVVSFRLVYDRETGKPKGYG
FCEYQDQETALSAMRNLNGR
EFSGRALRVDNAASEKNKEE
LKSLGTGA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 241 |
| 15N chemical shifts | 102 |
| 1H chemical shifts | 101 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 108 residues - 11965.345 Da.
| 1 | MET | ALA | GLY | LEU | THR | VAL | ARG | ASP | PRO | ALA | ||||
| 2 | VAL | ASP | ARG | SER | LEU | ARG | SER | VAL | PHE | VAL | ||||
| 3 | GLY | ASN | ILE | PRO | TYR | GLU | ALA | THR | GLU | GLU | ||||
| 4 | GLN | LEU | LYS | ASP | ILE | PHE | SER | GLU | VAL | GLY | ||||
| 5 | PRO | VAL | VAL | SER | PHE | ARG | LEU | VAL | TYR | ASP | ||||
| 6 | ARG | GLU | THR | GLY | LYS | PRO | LYS | GLY | TYR | GLY | ||||
| 7 | PHE | CYS | GLU | TYR | GLN | ASP | GLN | GLU | THR | ALA | ||||
| 8 | LEU | SER | ALA | MET | ARG | ASN | LEU | ASN | GLY | ARG | ||||
| 9 | GLU | PHE | SER | GLY | ARG | ALA | LEU | ARG | VAL | ASP | ||||
| 10 | ASN | ALA | ALA | SER | GLU | LYS | ASN | LYS | GLU | GLU | ||||
| 11 | LEU | LYS | SER | LEU | GLY | THR | GLY | ALA |
Samples:
sample_1: RNA Recognition Motif, [U-100% 13C; U-100% 15N], 980 uM
sample_conditions_1: ionic strength: 10 mM; pH: 6; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNmr Analysis v2.4.2, CCPN - chemical shift assignment
NMRDraw v9.6, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, peak picking
CS-ROSETTA, Shen, Vernon, Baker and Bax - refinement, structure calculation
NMR spectrometers:
- Agilent DD2 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks