BMRB Entry 30571

Title:
Solution structure of POS-1, a CCCH-type Tandem Zinc Finger protein from C. elegans
Deposition date:
2019-02-14
Original release date:
2020-10-09
Authors:
Ertekin, A.; Massi, F.
Citation:

Citation: Ertekin, A.; Massi, F.. "Solution structure of POS-1, a CCCH-type Tandem Zinc Finger protein from C. elegans"  .

Assembly members:

Assembly members:
entity_1, polymer, 78 residues, 8875.201 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: C. elegans   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pet21a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts322
15N chemical shifts67
1H chemical shifts482

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2ZINC ION, 12
3ZINC ION, 22

Entities:

Entity 1, unit_1 78 residues - 8875.201 Da.

1   SERASPALAPHELYSTHRALALEUCYSASP
2   ALATYRLYSARGSERGLNALACYSSERTYR
3   GLYASPGLNCYSARGPHEALAHISGLYVAL
4   HISGLULEUARGLEUPROMETASNPROARG
5   GLYARGASNHISPROLYSTYRLYSTHRVAL
6   LEUCYSASPLYSPHESERMETTHRGLYASN
7   CYSLYSTYRGLYTHRARGCYSGLNPHEILE
8   HISLYSILEVALASPGLYASNALA

Entity 2, ZINC ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: POS-1, [U-100% 13C; U-100% 15N], 0.4 mM; Tris-HCl pH 6.3 50 mM; potassium chloride 100 mM; Zinc Acetate 100 uM; DTT 1 mM; DSS 10 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.3; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
(HB)CB(CGCDCE)HEsample_1isotropicsample_conditions_1
(HB)CB(CGCD)HDsample_1isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

Sparky, Goddard - peak picking

NMR spectrometers:

  • Varian Agilent VNMRS 800 MHz
  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks