BMRB Entry 30474

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PDB ID: 6dmp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30474
MolProbity Validation Chart

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Title:
De Novo Design of a Protein Heterodimer with Specificity Mediated by Hydrogen Bond Networks
Deposition date:
2018-06-05
Original release date:
2018-12-13
Authors:
Chen, Z.; Flores-Solis, D.; Sgourakis, N.; Baker, D.
Citation:

Citation: Chen, Zibo; Boyken, Scott; Jia, Mengxuan; Busch, Florian; Flores-Solis, David; Bick, Matthew; Lu, Peilong; VanAernum, Zachary; Sahasrabuddhe, Aniruddha; Langan, Robert; Bermeo, Sherry; Brunette, T; Mulligan, Vikram Khipple; Carter, Lauren; DiMaio, Frank; Sgourakis, Nikolaos; Wysocki, Vicki; Baker, David. "Programmable design of orthogonal protein heterodimers."  Nature 565, 106-111 (2019).
PubMed: 30568301

Assembly members:

Assembly members:
entity_1, polymer, 77 residues, 9344.792 Da.
entity_2, polymer, 82 residues, 9798.264 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GTKEDILERQRKIIERAQEI HRRQQEILEELERIIRKPGS SEEAMKRMLKLLEESLRLLK ELLELSEESAQLLYEQR
entity_2: TEKRLLEEAERAHREQKEII KKAQELHRRLEEIVRQSGSS EEAKKEAKKILEEIRELSKR SLELLREILYLSQEQKGSLV PR

Data sets:
Data typeCount
13C chemical shifts625
15N chemical shifts146
1H chemical shifts1028

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 77 residues - 9344.792 Da.

1   GLYTHRLYSGLUASPILELEUGLUARGGLN
2   ARGLYSILEILEGLUARGALAGLNGLUILE
3   HISARGARGGLNGLNGLUILELEUGLUGLU
4   LEUGLUARGILEILEARGLYSPROGLYSER
5   SERGLUGLUALAMETLYSARGMETLEULYS
6   LEULEUGLUGLUSERLEUARGLEULEULYS
7   GLULEULEUGLULEUSERGLUGLUSERALA
8   GLNLEULEUTYRGLUGLNARG

Entity 2, entity_2 82 residues - 9798.264 Da.

1   THRGLULYSARGLEULEUGLUGLUALAGLU
2   ARGALAHISARGGLUGLNLYSGLUILEILE
3   LYSLYSALAGLNGLULEUHISARGARGLEU
4   GLUGLUILEVALARGGLNSERGLYSERSER
5   GLUGLUALALYSLYSGLUALALYSLYSILE
6   LEUGLUGLUILEARGGLULEUSERLYSARG
7   SERLEUGLULEULEUARGGLUILELEUTYR
8   LEUSERGLNGLUGLNLYSGLYSERLEUVAL
9   PROARG

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