BMRB Entry 30450

Click here to enlarge.

PDB ID: 6czt
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30450
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
CS-rosetta determined structures of the N-terminal domain of AlgF from P. aeruginosa
Deposition date:
2018-04-09
Original release date:
2019-04-09
Authors:
Tammam, S.; Howell, P.
Citation:

Citation: Low, K.; Tammam, S.; Whitfield, G.; Riley, L.; Weadge, J.; Caldwell, S.; Baker, P.; Chong, P.; Walvoort, M.; Kitova, E.; Grant, T.; Snell, E.; Klassen, J.; Codee, J.; Howell, P.. "Pseudomonas aeruginosa AlgF is an adaptor protein required for acetylation of the alginate exopolysaccharide"  .

Assembly members:

Assembly members:
P. aeruginosa AlgF, polymer, 196 residues, 20917.729 Da.

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 208964   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
P. aeruginosa AlgF: MEGALYGPQAPKGSAFVRAY NAGNSELDVSVGSTSLNDVA PLGSSDFKFLPPGSYTAQVG QQSLPVKLDPDSYYTLVSQP GGKPQLVAEPPFKNKQKALV RVQNLSGSKLTLKTADGKTD VVKDVGPQSHGDREINPVKV NLALFDGSKKVSDLKPVTLA RGEVVCLYVTGSGGKLAPVW VKRPVKADLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts815
15N chemical shifts190
1H chemical shifts1317

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1P. aeruginosa AlgF1

Entities:

Entity 1, P. aeruginosa AlgF 196 residues - 20917.729 Da.

1   METGLUGLYALALEUTYRGLYPROGLNALA
2   PROLYSGLYSERALAPHEVALARGALATYR
3   ASNALAGLYASNSERGLULEUASPVALSER
4   VALGLYSERTHRSERLEUASNASPVALALA
5   PROLEUGLYSERSERASPPHELYSPHELEU
6   PROPROGLYSERTYRTHRALAGLNVALGLY
7   GLNGLNSERLEUPROVALLYSLEUASPPRO
8   ASPSERTYRTYRTHRLEUVALSERGLNPRO
9   GLYGLYLYSPROGLNLEUVALALAGLUPRO
10   PROPHELYSASNLYSGLNLYSALALEUVAL
11   ARGVALGLNASNLEUSERGLYSERLYSLEU
12   THRLEULYSTHRALAASPGLYLYSTHRASP
13   VALVALLYSASPVALGLYPROGLNSERHIS
14   GLYASPARGGLUILEASNPROVALLYSVAL
15   ASNLEUALALEUPHEASPGLYSERLYSLYS
16   VALSERASPLEULYSPROVALTHRLEUALA
17   ARGGLYGLUVALVALCYSLEUTYRVALTHR
18   GLYSERGLYGLYLYSLEUALAPROVALTRP
19   VALLYSARGPROVALLYSALAASPLEUGLU
20   HISHISHISHISHISHIS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks