BMRB Entry 30368

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PDB ID: 6bgh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30368
MolProbity Validation Chart

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Title:
Solution NMR structure of Brd3 ET domain bound to Brg1 peptide
Deposition date:
2017-10-28
Original release date:
2018-03-14
Authors:
Szyszka, T.; Wai, D.; Mackay, J.
Citation:

Citation: Wai, Dorothy; Szyszka, Taylor; Campbell, Amy; Kwong, Cherry; Wilkinson-White, Lorna; Silva, Ana; Low, Jason; Kwan, Ann; Gamsjaeger, Roland; Chalmers, James; Patrick, Wayne; Lu, Bin; Vakoc, Christopher; Blobel, Gerd; Mackay, Joel. "The BRD3 ET domain recognizes a short peptide motif through a mechanism that is conserved across chromatin remodelers and transcriptional regulators"  J. Biol. Chem. 293, 7160-7175 (2018).
PubMed: 29567837

Assembly members:

Assembly members:
entity_1, polymer, 87 residues, 10179.387 Da.
entity_2, polymer, 12 residues, 1417.829 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ASASYDSEEEEEGLPMSYDE KRQLSLDINRLPGEKLGRVV HIIQSREPSLRDSNPDEIEI DFETLKPTTLRELERYVKSC LQKKQRK
entity_2: RSVKVKIKLGRK

Data sets:
Data typeCount
13C chemical shifts352
15N chemical shifts79
1H chemical shifts669

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 87 residues - 10179.387 Da.

1   ALASERALASERTYRASPSERGLUGLUGLU
2   GLUGLUGLYLEUPROMETSERTYRASPGLU
3   LYSARGGLNLEUSERLEUASPILEASNARG
4   LEUPROGLYGLULYSLEUGLYARGVALVAL
5   HISILEILEGLNSERARGGLUPROSERLEU
6   ARGASPSERASNPROASPGLUILEGLUILE
7   ASPPHEGLUTHRLEULYSPROTHRTHRLEU
8   ARGGLULEUGLUARGTYRVALLYSSERCYS
9   LEUGLNLYSLYSGLNARGLYS

Entity 2, entity_2 12 residues - 1417.829 Da.

1   ARGSERVALLYSVALLYSILELYSLEUGLY
2   ARGLYS

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