BMRB Entry 30300

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PDB ID: 5vzm
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30300
MolProbity Validation Chart

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Title:
Solution NMR structure of human Rev1 (932-1039) in complex with ubiquitin
Deposition date:
2017-05-29
Original release date:
2018-05-17
Authors:
Cui, G.; Botuyan, M.; Mer, G.
Citation:

Citation: Cui, G.; Botuyan, M.; Mer, G.. "Solution NMR structure of human Rev1 (932-1039) in complex with ubiquitin"  J. Mol. Biol. 430, 2042-2050 (2018).
PubMed: 29778604

Assembly members:

Assembly members:
entity_1, polymer, 76 residues, 8576.831 Da.
entity_2, polymer, 111 residues, 11985.354 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSUb

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG
entity_2: GHMPSPSQLDQSVLEALPPD LREQVEQVCAVQQAESHGDK KKEPVNGCNTGILPQPVGTV LLQIPEPQESNSDAGINLIA LPAFSQVDPEVFAALPAELQ RELKAAYDQRQ

Data sets:
Data typeCount
13C chemical shifts802
15N chemical shifts173
1H chemical shifts1289

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 76 residues - 8576.831 Da.

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Entity 2, entity_2 111 residues - 11985.354 Da.

1   GLYHISMETPROSERPROSERGLNLEUASP
2   GLNSERVALLEUGLUALALEUPROPROASP
3   LEUARGGLUGLNVALGLUGLNVALCYSALA
4   VALGLNGLNALAGLUSERHISGLYASPLYS
5   LYSLYSGLUPROVALASNGLYCYSASNTHR
6   GLYILELEUPROGLNPROVALGLYTHRVAL
7   LEULEUGLNILEPROGLUPROGLNGLUSER
8   ASNSERASPALAGLYILEASNLEUILEALA
9   LEUPROALAPHESERGLNVALASPPROGLU
10   VALPHEALAALALEUPROALAGLULEUGLN
11   ARGGLULEULYSALAALATYRASPGLNARG
12   GLN

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