BMRB Entry 30234

Title:
Representative 1-conformer ensembles of K27-linked Ub2 from RDC data
Deposition date:
2017-01-18
Original release date:
2017-10-20
Authors:
Castaneda, C.; Fushman, D.
Citation:

Citation: Castaneda, C.; Dixon, E.; Walker, O.; Chaturvedi, A.; Nakasone, M.; Curtis, J.; Reed, M.; Krueger, S.; Cropp, T.; Fushman, D.. "Linkage via K27 Bestows Ubiquitin Chains with Unique Properties among Polyubiquitins."  Structure 24, 423-436 (2016).
PubMed: 26876099

Assembly members:

Assembly members:
entity_1, polymer, 76 residues, 8576.831 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts140
1H chemical shifts140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1, 11
2entity_1, 21

Entities:

Entity 1, entity_1, 1 76 residues - 8576.831 Da.

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Samples:

sample_1: K27-diubiquitin, Distal Ub is 15N-labelled Proximal Ub is not enriched, [U-99% 15N], 150 uM

sample_2: K27-diubiquitin, Distal Ub is not enriched Proximal Ub is 15N-labelled, [U-99% 15N], 150 uM

sample_conditions_1: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 296 K

sample_conditions_2: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_1anisotropicsample_conditions_2
2D 1H-15N HSQCsample_2anisotropicsample_conditions_2

Software:

ARMOR v1.1, Berlin K, Castaneda CA, Fushman D. - refinement

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AvanceIII 800 MHz
  • Bruker AvanceIII 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks