BMRB Entry 30192

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PDB ID: 5tmx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30192
MolProbity Validation Chart

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Title:
Solution Structure of SinI, antagonist to the master biofilm-regulator SinR in Bacillus subtilis
Deposition date:
2016-10-13
Original release date:
2017-10-19
Authors:
Draughn, G.; Bobay, B.; Stowe, S.; Thompson, R.; Cavanagh, J.
Citation:

Citation: Milton, Morgan; Draughn, G Logan; Bobay, Benjamin; Stowe, Sean; Olson, Andrew; Feldmann, Erik; Thompson, Richele; Myers, Katherine; Santoro, Michael; Kearns, Daniel; Cavanagh, John. "The Solution Structures and Interaction of SinR and SinI: Elucidating the Mechanism of Action of the Master Regulator Switch for Biofilm Formation in Bacillus subtilis"  J. Mol. Biol. 432, 343-357 (2020).
PubMed: 31493408

Assembly members:

Assembly members:
Protein SinI, polymer, 63 residues, 7184.149 Da.

Natural source:

Natural source:   Common Name: firmicutes   Taxonomy ID: 224308   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Protein SinI: GSHMASMKNAKQEHFELDQE WVELMVEAKEANISPEEIRK YLLLNKKSAHPGPAARSHTV NPF

Data sets:
Data typeCount
13C chemical shifts252
15N chemical shifts51
1H chemical shifts389

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1, chain 11
2entity_1, chain 21

Entities:

Entity 1, entity_1, chain 1 63 residues - 7184.149 Da.

1   GLYSERHISMETALASERMETLYSASNALA
2   LYSGLNGLUHISPHEGLULEUASPGLNGLU
3   TRPVALGLULEUMETVALGLUALALYSGLU
4   ALAASNILESERPROGLUGLUILEARGLYS
5   TYRLEULEULEUASNLYSLYSSERALAHIS
6   PROGLYPROALAALAARGSERHISTHRVAL
7   ASNPROPHE

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