BMRB Entry 30159
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PDB ID: 5t1o
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30159
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Strickland, Madeleine; Stanley, Ann Marie; Wang, Guangshun; Botos, Istvan; Schwieters, Charles; Buchanan, Susan; Peterkofsky, Alan; Tjandra, Nico. "Structure of the NPr:EINNtr Complex: Mechanism for Specificity in Paralogous Phosphotransferase Systems" Structure 24, 2127-2137 (2016).
PubMed: 27839951
Assembly members:
entity_1, polymer, 85 residues, 9254.570 Da.
entity_2, polymer, 256 residues, 28380.070 Da.
Natural source: Common Name: E. coli Taxonomy ID: 83334 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Plasmid
Entity Sequences (FASTA):
entity_1: MTVKQTVEITNKLGMHARPA
MKLFELMQGFDAEVLLRNDE
GTEAEANSVIALLMLDSAKG
RQIEVEATGPQEEEALAAVI
ALFNS
entity_2: GRIRALPAAPGVAIAEGWQD
ATLPLMEQVYQASTLDPALE
RERLTGALEEAANEFRRYSK
RFAAGAQKETAAIFDLYSHL
LSDTRLRRELFAEVDKGSVA
EWAVKTVIEKFAEQFAALSD
NYLKERAGDLRALGQRLLFH
LDDANQGPNAWPERFILVAD
ELSATTLAELPQDRLVGVVV
RDGAANSQAAIMVRALGIPT
VMGADIQPSVLHRRTLIVDG
YRGELLVDPEPVLLQEYQRL
ISEEIELSRLAEDDVN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 821 |
| 15N chemical shifts | 285 |
| 1H chemical shifts | 285 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 2 |
Entities:
Entity 1, entity_1 85 residues - 9254.570 Da.
| 1 | MET | THR | VAL | LYS | GLN | THR | VAL | GLU | ILE | THR | ||||
| 2 | ASN | LYS | LEU | GLY | MET | HIS | ALA | ARG | PRO | ALA | ||||
| 3 | MET | LYS | LEU | PHE | GLU | LEU | MET | GLN | GLY | PHE | ||||
| 4 | ASP | ALA | GLU | VAL | LEU | LEU | ARG | ASN | ASP | GLU | ||||
| 5 | GLY | THR | GLU | ALA | GLU | ALA | ASN | SER | VAL | ILE | ||||
| 6 | ALA | LEU | LEU | MET | LEU | ASP | SER | ALA | LYS | GLY | ||||
| 7 | ARG | GLN | ILE | GLU | VAL | GLU | ALA | THR | GLY | PRO | ||||
| 8 | GLN | GLU | GLU | GLU | ALA | LEU | ALA | ALA | VAL | ILE | ||||
| 9 | ALA | LEU | PHE | ASN | SER |
Entity 2, entity_2 256 residues - 28380.070 Da.
| 1 | GLY | ARG | ILE | ARG | ALA | LEU | PRO | ALA | ALA | PRO | ||||
| 2 | GLY | VAL | ALA | ILE | ALA | GLU | GLY | TRP | GLN | ASP | ||||
| 3 | ALA | THR | LEU | PRO | LEU | MET | GLU | GLN | VAL | TYR | ||||
| 4 | GLN | ALA | SER | THR | LEU | ASP | PRO | ALA | LEU | GLU | ||||
| 5 | ARG | GLU | ARG | LEU | THR | GLY | ALA | LEU | GLU | GLU | ||||
| 6 | ALA | ALA | ASN | GLU | PHE | ARG | ARG | TYR | SER | LYS | ||||
| 7 | ARG | PHE | ALA | ALA | GLY | ALA | GLN | LYS | GLU | THR | ||||
| 8 | ALA | ALA | ILE | PHE | ASP | LEU | TYR | SER | HIS | LEU | ||||
| 9 | LEU | SER | ASP | THR | ARG | LEU | ARG | ARG | GLU | LEU | ||||
| 10 | PHE | ALA | GLU | VAL | ASP | LYS | GLY | SER | VAL | ALA | ||||
| 11 | GLU | TRP | ALA | VAL | LYS | THR | VAL | ILE | GLU | LYS | ||||
| 12 | PHE | ALA | GLU | GLN | PHE | ALA | ALA | LEU | SER | ASP | ||||
| 13 | ASN | TYR | LEU | LYS | GLU | ARG | ALA | GLY | ASP | LEU | ||||
| 14 | ARG | ALA | LEU | GLY | GLN | ARG | LEU | LEU | PHE | HIS | ||||
| 15 | LEU | ASP | ASP | ALA | ASN | GLN | GLY | PRO | ASN | ALA | ||||
| 16 | TRP | PRO | GLU | ARG | PHE | ILE | LEU | VAL | ALA | ASP | ||||
| 17 | GLU | LEU | SER | ALA | THR | THR | LEU | ALA | GLU | LEU | ||||
| 18 | PRO | GLN | ASP | ARG | LEU | VAL | GLY | VAL | VAL | VAL | ||||
| 19 | ARG | ASP | GLY | ALA | ALA | ASN | SER | GLN | ALA | ALA | ||||
| 20 | ILE | MET | VAL | ARG | ALA | LEU | GLY | ILE | PRO | THR | ||||
| 21 | VAL | MET | GLY | ALA | ASP | ILE | GLN | PRO | SER | VAL | ||||
| 22 | LEU | HIS | ARG | ARG | THR | LEU | ILE | VAL | ASP | GLY | ||||
| 23 | TYR | ARG | GLY | GLU | LEU | LEU | VAL | ASP | PRO | GLU | ||||
| 24 | PRO | VAL | LEU | LEU | GLN | GLU | TYR | GLN | ARG | LEU | ||||
| 25 | ILE | SER | GLU | GLU | ILE | GLU | LEU | SER | ARG | LEU | ||||
| 26 | ALA | GLU | ASP | ASP | VAL | ASN |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks