BMRB Entry 30092

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PDB ID: 5jyu
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30092
MolProbity Validation Chart

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Title:
NMR structure of pseudo receiver domain of CikA from Thermosynechococcus elongatus
Deposition date:
2016-05-15
Original release date:
2017-03-23
Authors:
Tseng, Roger; Wang, Andy
Citation:

Citation: Tseng, Roger; Goularte, Nicolette; Chavan, Archana; Luu, Jansen; Cohen, Susan; Chang, Yong-Gang; Heisler, Joel; Li, Sheng; Michael, Alicia; Tripathi, Sarvind; Golden, Susan; LiWang, Andy; Partch, Carrie. "Structural basis of the day-night transition in a bacterial circadian clock"  Science 355, 1174-1180 (2017).
PubMed: 28302851

Assembly members:

Assembly members:
Two-component sensor histidine kinase, polymer, 117 residues, 12899.736 Da.

Natural source:

Natural source:   Common Name: cyanobacteria   Taxonomy ID: 197221   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermosynechococcus elongatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Two-component sensor histidine kinase: EGRIVLVSEDEATSTLICSI LTTAGYQVIWLVDGEVERLL ALTPIAVLLAEPFSYGDVQE LVDQLRQRCTPEQLKIFILG SKGNYQGVDRYIPLPIHPES FLQQVTMGLTSLATSAQ

Data sets:
Data typeCount
13C chemical shifts488
15N chemical shifts110
1H chemical shifts799

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 117 residues - 12899.736 Da.

1   GLUGLYARGILEVALLEUVALSERGLUASP
2   GLUALATHRSERTHRLEUILECYSSERILE
3   LEUTHRTHRALAGLYTYRGLNVALILETRP
4   LEUVALASPGLYGLUVALGLUARGLEULEU
5   ALALEUTHRPROILEALAVALLEULEUALA
6   GLUPROPHESERTYRGLYASPVALGLNGLU
7   LEUVALASPGLNLEUARGGLNARGCYSTHR
8   PROGLUGLNLEULYSILEPHEILELEUGLY
9   SERLYSGLYASNTYRGLNGLYVALASPARG
10   TYRILEPROLEUPROILEHISPROGLUSER
11   PHELEUGLNGLNVALTHRMETGLYLEUTHR
12   SERLEUALATHRSERALAGLN

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