BMRB Entry 30010
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PDB ID: 5i22
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR30010
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Tossavainen, H.; Aitio, O.; Hellman, M.; Saksela, K.; Permi, P.. "Structural Basis of the High Affinity Interaction between the Alphavirus Nonstructural Protein-3 (nsP3) and the SH3 Domain of Amphiphysin-2" J. Biol. Chem. 291, 16307-16317 (2016).
PubMed: 27268056
Assembly members:
entity_1, polymer, 81 residues, 9356.412 Da.
entity_2, polymer, 17 residues, 1938.266 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: GRLDLPPGFMFKVQAQHDYT
ATDTDELQLKAGDVVLVIPF
QNPEEQDEGWLMGVKESDWN
QHKELEKCRGVFPENFTERV
P
entity_2: STVPVAPPRRRRGRNLT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 334 |
| 15N chemical shifts | 105 |
| 1H chemical shifts | 704 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 2 |
Entities:
Entity 1, entity_1 81 residues - 9356.412 Da.
| 1 | GLY | ARG | LEU | ASP | LEU | PRO | PRO | GLY | PHE | MET | ||||
| 2 | PHE | LYS | VAL | GLN | ALA | GLN | HIS | ASP | TYR | THR | ||||
| 3 | ALA | THR | ASP | THR | ASP | GLU | LEU | GLN | LEU | LYS | ||||
| 4 | ALA | GLY | ASP | VAL | VAL | LEU | VAL | ILE | PRO | PHE | ||||
| 5 | GLN | ASN | PRO | GLU | GLU | GLN | ASP | GLU | GLY | TRP | ||||
| 6 | LEU | MET | GLY | VAL | LYS | GLU | SER | ASP | TRP | ASN | ||||
| 7 | GLN | HIS | LYS | GLU | LEU | GLU | LYS | CYS | ARG | GLY | ||||
| 8 | VAL | PHE | PRO | GLU | ASN | PHE | THR | GLU | ARG | VAL | ||||
| 9 | PRO |
Entity 2, entity_2 17 residues - 1938.266 Da.
| 1 | SER | THR | VAL | PRO | VAL | ALA | PRO | PRO | ARG | ARG | ||||
| 2 | ARG | ARG | GLY | ARG | ASN | LEU | THR |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks