BMRB Entry 27785

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27785

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Title:
1H, 13C and 15N resonance assignments for the first and second CUE domains from chromatin remodeler SMARCAD1
Deposition date:
2019-02-13
Original release date:
2019-04-12
Authors:
Redfield, Christina; Biasutto, Antonio; Mancini, Erika; West, Phillip
Citation:

Citation: Biasutto, Antonio; West, Phillip; Mancini, Erika; Redfield, Christina. "1H, 13C and 15N resonance assignments for the tandem CUE domains from chromatin remodeler SMARCAD1"  Biomol. NMR Assignments 13, 261-265 (2019).
PubMed: 30919308

Assembly members:

Assembly members:
dCUE, polymer, 166 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-DUET-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dCUE: MGSSHHHHHHSQDPDISELE DLSELEDLKDAKLQTLKELF PQRSDNDLLKLIESTSTMDG AIAAALLMFGDAGGGPRKRK LSSSSEPYEEDEFNDDQSIK KTRLDHGEESNESAESSNNW EKQESIVLKLQKEFPNFDKQ ELREVLKEHEWMYTEALESL KVFAED

Data sets:
Data typeCount
13C chemical shifts550
15N chemical shifts139
1H chemical shifts831

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1dCUE1

Entities:

Entity 1, dCUE 166 residues - Formula weight is not available

Residues 130-143 are a non-native N-terminal His6 tag and a thrombin cleavage site.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERGLNASPPROASPILESERGLULEUGLU
3   ASPLEUSERGLULEUGLUASPLEULYSASP
4   ALALYSLEUGLNTHRLEULYSGLULEUPHE
5   PROGLNARGSERASPASNASPLEULEULYS
6   LEUILEGLUSERTHRSERTHRMETASPGLY
7   ALAILEALAALAALALEULEUMETPHEGLY
8   ASPALAGLYGLYGLYPROARGLYSARGLYS
9   LEUSERSERSERSERGLUPROTYRGLUGLU
10   ASPGLUPHEASNASPASPGLNSERILELYS
11   LYSTHRARGLEUASPHISGLYGLUGLUSER
12   ASNGLUSERALAGLUSERSERASNASNTRP
13   GLULYSGLNGLUSERILEVALLEULYSLEU
14   GLNLYSGLUPHEPROASNPHEASPLYSGLN
15   GLULEUARGGLUVALLEULYSGLUHISGLU
16   TRPMETTYRTHRGLUALALEUGLUSERLEU
17   LYSVALPHEALAGLUASP

Related Database Links:

UNP Q9H4L7
AlphaFold Q9ULU7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks