BMRB Entry 27733

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27733

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Title:
15N, 13C and 1H backbone resonance assignments of the FKBP12 protein from Aspergillus fumigatus bound to FK506
Deposition date:
2018-12-20
Original release date:
2019-03-08
Authors:
Gobeil, Sophie; Bobay, Benjamin; Spicer, Leonard; Venters, Ronald
Citation:

Citation: Gobeil, Sophie; Bobay, Benjamin; Spicer, Leonard; Venters, Ronald. "15N, 13C and 1H resonance assignments of FKBP12 proteins from the pathogenic fungi Mucor circinelloides and Aspergillus fumigatus"  Biomol. NMR Assignments 13, 207-212 (2019).
PubMed: 30707421

Assembly members:

Assembly members:
FKBP12, polymer, 112 residues, 12000 Da.
entity_FK5, non-polymer, 804.018 Da.

Natural source:

Natural source:   Common Name: Aspergillus fumigatus   Taxonomy ID: 746128   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Aspergillus fumigatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FKBP12: MGVTKELKSPGNGVDFPKKG DFVTIHYTGRLTDGSKFDSS VDRNEPFQTQIGTGRVIKGW DEGVPQMSLGEKAVLTITPD YGYGARGFPPVIPGNSTLIF EVELLGINNKRA

Data sets:
Data typeCount
13C chemical shifts316
15N chemical shifts103
1H chemical shifts215

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FKBP121
2Tacrolimus2

Entities:

Entity 1, FKBP12 112 residues - 12000 Da.

1   METGLYVALTHRLYSGLULEULYSSERPRO
2   GLYASNGLYVALASPPHEPROLYSLYSGLY
3   ASPPHEVALTHRILEHISTYRTHRGLYARG
4   LEUTHRASPGLYSERLYSPHEASPSERSER
5   VALASPARGASNGLUPROPHEGLNTHRGLN
6   ILEGLYTHRGLYARGVALILELYSGLYTRP
7   ASPGLUGLYVALPROGLNMETSERLEUGLY
8   GLULYSALAVALLEUTHRILETHRPROASP
9   TYRGLYTYRGLYALAARGGLYPHEPROPRO
10   VALILEPROGLYASNSERTHRLEUILEPHE
11   GLUVALGLULEULEUGLYILEASNASNLYS
12   ARGALA

Entity 2, Tacrolimus - C44 H69 N O12 - 804.018 Da.

1   FK5

Related Database Links:

NCBI XP_751096

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks