BMRB Entry 27595

Assembly:

Entities:

Entity 1, ARID monomer 115 residues - Formula weight is not available

Residues 107-115 represent a non-native residues L, E and six H of affinity tag. This is the ARID domain of BAF200

Samples:

sample_1: BAF200 ARID, [U-99% 15N], 0.5 ± 0.1 mM; D2O, [U-2H], 10 ± 0.1 %; sodium phosphate 50 ± 0.1 mM; sodium chloride 50 ± 0.1 mM; DTT 2 ± 0.1 mM; H2O 90 ± 0.1 %

sample_2: BAF200 ARID, [U-99% 13C; U-99% 15N], 0.6 ± 0.1 mM; D2O, [U-2H], 10 ± 0.1 %; sodium phosphate 50 ± 0.1 mM; sodium chloride 50 ± 0.1 mM; DTT 2 ± 0.1 mM; H2O 90 ± 0.1 %

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

VNMRJ, Varian - collection, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, chemical shift calculation, data analysis, peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis, dihedral angles

NMR spectrometers:

• Agilent DRX 600 MHz