BMRB Entry 27574
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27574
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Henen, Morkos; Mahlawat, Pardeep; Zwieb, Christian; Kodali, Ravi; Hanna, Ramsey; Krzysiak, Troy; Ilangovan, Udayar; Cano, Kristin; Hinck, Garrett; Vonberg, Morkos; McCabe, Megan; Hinck, Andrew. "TGF-b2 uses the concave surface of its extended finger region to bind betaglycan's ZP domain via three residues specific to TGF-b and Inhibin-a" J. Biol. Chem. 294, 3065-3080 (2019).
PubMed: 30598510
Assembly members:
TGF-b2, polymer, 112 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32a
Entity Sequences (FASTA):
TGF-b2: ALDAAYCFRNVQDNCCLRPL
YIDFKRDLGWKWIHEPKGYN
ANFCAGACPYLWSSDTQHSK
VLSLYNTINPEASASPCCVS
QDLEPLTILYYIGKTPKIEQ
LSNMIVKSCKCS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 383 |
| 15N chemical shifts | 92 |
| 1H chemical shifts | 499 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TGF-b2, chain 1 | 1 |
| 2 | TGF-b2, chain 2 | 1 |
Entities:
Entity 1, TGF-b2, chain 1 112 residues - Formula weight is not available
The sequence provided is that of the monomer, though the protein found in the NMR tube is a covalent (disulfide) linked homodimer. There is a single Arg to Lys substitution at position 60.
| 1 | ALA | LEU | ASP | ALA | ALA | TYR | CYS | PHE | ARG | ASN | ||||
| 2 | VAL | GLN | ASP | ASN | CYS | CYS | LEU | ARG | PRO | LEU | ||||
| 3 | TYR | ILE | ASP | PHE | LYS | ARG | ASP | LEU | GLY | TRP | ||||
| 4 | LYS | TRP | ILE | HIS | GLU | PRO | LYS | GLY | TYR | ASN | ||||
| 5 | ALA | ASN | PHE | CYS | ALA | GLY | ALA | CYS | PRO | TYR | ||||
| 6 | LEU | TRP | SER | SER | ASP | THR | GLN | HIS | SER | LYS | ||||
| 7 | VAL | LEU | SER | LEU | TYR | ASN | THR | ILE | ASN | PRO | ||||
| 8 | GLU | ALA | SER | ALA | SER | PRO | CYS | CYS | VAL | SER | ||||
| 9 | GLN | ASP | LEU | GLU | PRO | LEU | THR | ILE | LEU | TYR | ||||
| 10 | TYR | ILE | GLY | LYS | THR | PRO | LYS | ILE | GLU | GLN | ||||
| 11 | LEU | SER | ASN | MET | ILE | VAL | LYS | SER | CYS | LYS | ||||
| 12 | CYS | SER |
Related Database Links:
| NCBI | NP_003229.1 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks