BMRB Entry 27511

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27511

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Title:
Backbone resonance assignment of a Huwe1 HECT domain C-lobe mutant
Deposition date:
2018-06-06
Original release date:
2018-07-12
Authors:
Wiesner, Silke; Jaeckl, Magnus; Stollmaier, Carsten; Strohaeker, Timo; Hyz, Karolina; Maspero, Elena; Polo, Simona
Citation:

Citation: Jaeckl, Magnus; Stollmaier, Carsten; Strohaeker, Timo; Hyz, Karolina; Maspero, Elena; Polo, Simona; Wiesner, Silke. "Beta-Sheet Augmentation Is a Conserved Mechanism of Priming HECT E3 Ligases for Ubiquitin Ligation"  J. Mol. Biol. 430, 3218-3233 (2018).
PubMed: 29964046

Assembly members:

Assembly members:
clobe, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-M11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
clobe: GAMGLPTIDIDDLKSNTEYH KYQSNSIQIQWFWRALRSFD QADRAKFLQFVTGTSKVPLQ GFAALEGMNGIQKFQIHRDD RSTDRLPSAHTCFNQLDLPA YESFEKLRHMLLLAIQENSE GFGLA

Data sets:
Data typeCount
13C chemical shifts235
15N chemical shifts118
1H chemical shifts118

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1clobe1

Entities:

Entity 1, clobe 124 residues - Formula weight is not available

N-terminal GAMG is a cloning artefact. The construct contains a C378N mutation.

1   GLYALAMETGLYLEUPROTHRILEASPILE
2   ASPASPLEULYSSERASNTHRGLUTYRHIS
3   LYSTYRGLNSERASNSERILEGLNILEGLN
4   TRPPHETRPARGALALEUARGSERPHEASP
5   GLNALAASPARGALALYSPHELEUGLNPHE
6   VALTHRGLYTHRSERLYSVALPROLEUGLN
7   GLYPHEALAALALEUGLUGLYMETASNGLY
8   ILEGLNLYSPHEGLNILEHISARGASPASP
9   ARGSERTHRASPARGLEUPROSERALAHIS
10   THRCYSPHEASNGLNLEUASPLEUPROALA
11   TYRGLUSERPHEGLULYSLEUARGHISMET
12   LEULEULEUALAILEGLNGLUASNSERGLU
13   GLYPHEGLYLEUALA

Related Database Links:

NCBI NP_113584.3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks