BMRB Entry 27459

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27459

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Title:
side-chain methyl order parameters for Zn(II)-bound CzrA L34A mutant at 25C-40C
Deposition date:
2018-04-20
Original release date:
2018-09-18
Authors:
Capdevila, Daiana; Edmonds, Katherine; Wu, Hongwei; Giedroc, David
Citation:

Citation: Capdevila, Daiana; Edmonds, Katherine; Campanello, Gregory; Wu, Hongwei; Gonzalez-Gutierrez, Giovanni; Giedroc, David. "Functional Role of Solvent Entropy and Conformational Entropy of Metal Binding in a Dynamically Driven Allosteric System"  J. Am. Chem. Soc. 140, 9108-9119 (2018).
PubMed: 29953213

Assembly members:

Assembly members:
CzrA_chain, polymer, 106 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CzrA_chain: MAEQYSEINTDTLERVTEIF KALGDYNRIRIMEALSVSEA SVGHISHQLNLSQSNVSHQL KLLKSVHLVKAKRQGQSMIY SLDDIHVATMLKQAIHHANH PKESGL

Data sets:
Data typeCount
13C chemical shifts260
15N chemical shifts99
1H chemical shifts379
order parameters224

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CzrA11
2CzrA21
3Zn12
4Zn22

Entities:

Entity 1, CzrA1 106 residues - Formula weight is not available

1   METALAGLUGLNTYRSERGLUILEASNTHR
2   ASPTHRLEUGLUARGVALTHRGLUILEPHE
3   LYSALALEUGLYASPTYRASNARGILEARG
4   ILEMETGLUALALEUSERVALSERGLUALA
5   SERVALGLYHISILESERHISGLNLEUASN
6   LEUSERGLNSERASNVALSERHISGLNLEU
7   LYSLEULEULYSSERVALHISLEUVALLYS
8   ALALYSARGGLNGLYGLNSERMETILETYR
9   SERLEUASPASPILEHISVALALATHRMET
10   LEULYSGLNALAILEHISHISALAASNHIS
11   PROLYSGLUSERGLYLEU

Entity 2, Zn1 - Zn - 65.409 Da.

1   ZN

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