BMRB Entry 27458

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27458

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Title:
Backbone and Side-chain Chemical Shift Assignments for MarH
Deposition date:
2018-04-20
Original release date:
2018-06-20
Authors:
Liu, Bin; Hu, Kaifeng
Citation:

Citation: Liu, Bin; Fang, Shiqi; Ma, Xiaofang; Bai, Zhiqiang; Hu, Kaifeng. "Backbone and side-chain chemical shift assignments of MarH, a critical intermediary epimerase for biosynthesis of Maremycins in Streptomyces"  Biomol. NMR Assign. 12, 335-338 (2018).
PubMed: 30054867

Assembly members:

Assembly members:
MarH, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Streptomyces   Taxonomy ID: 1883   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MarH: GSRPADPEIVEGLPIPLAVA GHHQPAPFYLTADMFGGLPV QLAGGELSTLVGKPVAAPHT HPVDELYLLVSPNKGGARIE VQLDGRRHELLSPAVMRIPA GSEHCFLTLEAEVGSYCFGI LLGDRL

Data sets:
Data typeCount
13C chemical shifts489
15N chemical shifts116
1H chemical shifts786

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MarH dimer, subunit 11
2MarH dimer, subunit 21

Entities:

Entity 1, MarH dimer, subunit 1 126 residues - Formula weight is not available

1   GLYSERARGPROALAASPPROGLUILEVAL
2   GLUGLYLEUPROILEPROLEUALAVALALA
3   GLYHISHISGLNPROALAPROPHETYRLEU
4   THRALAASPMETPHEGLYGLYLEUPROVAL
5   GLNLEUALAGLYGLYGLULEUSERTHRLEU
6   VALGLYLYSPROVALALAALAPROHISTHR
7   HISPROVALASPGLULEUTYRLEULEUVAL
8   SERPROASNLYSGLYGLYALAARGILEGLU
9   VALGLNLEUASPGLYARGARGHISGLULEU
10   LEUSERPROALAVALMETARGILEPROALA
11   GLYSERGLUHISCYSPHELEUTHRLEUGLU
12   ALAGLUVALGLYSERTYRCYSPHEGLYILE
13   LEULEUGLYASPARGLEU

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks