BMRB Entry 27435

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PDB ID: 6e3c
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27435
MolProbity Validation Chart

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Title:
NMR assignments of Decorator, a phage-cementing 15 KDa monomer
Deposition date:
2018-03-28
Original release date:
2018-09-19
Authors:
Newcomer, Rebecca; Belato, Helen; Teschke, Carolyn; Alexandrescu, Andrei
Citation:

Citation: Newcomer, Rebecca; Belato, Helen; Teschke, Carolyn; Alexandrescu, Andrei. "NMR assignments for monomeric phage L decoration protein"  Biomol. NMR Assignments 12, 339-343 (2018).
PubMed: 30109462

Assembly members:

Assembly members:
Decorator, polymer, 137 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Enterobacteria phage L   Taxonomy ID: 45441   Superkingdom: Viruses   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Decorator: GSHMANPNFTPSWPLYKDAD GVYVSALPIKAIKYANDGSA NAEFDGPYADQYMSAQTVAV FKPEVGGYLFRSQYGELLYM SKTAFEANYTSASGSVANAE TADKLSTARTITLTGAVTGS ASFDGSANVTIETTSGS

Data sets:
Data typeCount
13C chemical shifts460
15N chemical shifts141
1H chemical shifts739

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Decorator monomer1

Entities:

Entity 1, Decorator monomer 137 residues - Formula weight is not available

The residues 'gsh' are an artifact of thrombin cleavage. The actual sequence of Dec begins with 'M...', with M being residue 1.

1   GLYSERHISMETALAASNPROASNPHETHR
2   PROSERTRPPROLEUTYRLYSASPALAASP
3   GLYVALTYRVALSERALALEUPROILELYS
4   ALAILELYSTYRALAASNASPGLYSERALA
5   ASNALAGLUPHEASPGLYPROTYRALAASP
6   GLNTYRMETSERALAGLNTHRVALALAVAL
7   PHELYSPROGLUVALGLYGLYTYRLEUPHE
8   ARGSERGLNTYRGLYGLULEULEUTYRMET
9   SERLYSTHRALAPHEGLUALAASNTYRTHR
10   SERALASERGLYSERVALALAASNALAGLU
11   THRALAASPLYSLEUSERTHRALAARGTHR
12   ILETHRLEUTHRGLYALAVALTHRGLYSER
13   ALASERPHEASPGLYSERALAASNVALTHR
14   ILEGLUTHRTHRSERGLYSER

Related Database Links:

GB AY795968.1

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