BMRB Entry 27432

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PDB ID: 6g8o
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27432
MolProbity Validation Chart

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Title:
Solution structure of the cross-linked dimer of the SLy1 SAM domain S320C mutant
Deposition date:
2018-03-21
Original release date:
2019-01-25
Authors:
Kukuk, Laura; Dingley, Andrew; Koenig, Bernd
Citation:

Citation: Kukuk, Laura; Dingley, Andrew; Granzin, Joachim; Nagel-Steger, Luitgard; Thiagarajan-Rosenkranz, Pallavi; Ciupka, Daniel; Hanel, Karen; Batra-Safferling, Renu; Pacheco, Victor; Stoldt, Matthias; Pfeffer, Klaus; Beer-Hammer, Sandra; Willbold, Dieter; Koenig, Bernd. "Structure of the SLy1 SAM homodimer reveals a new interface for SAM domain self-association."  Sci. Rep. 9, 54-54 (2019).
PubMed: 30631134

Assembly members:

Assembly members:
Sly1_SAM, polymer, 69 residues, 7940.9134 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6p-2_SLYSAM_sh_C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sly1_SAM: GPKTLHELLERIGLEEHTST LLLNGYQTLEDFKELRETHL NELNIMDPQHRAKLLTAAEL LLDYDTGCE

Data sets:
Data typeCount
13C chemical shifts316
15N chemical shifts72
1H chemical shifts504

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SAM A1
2SAM B1

Entities:

Entity 1, SAM A 69 residues - 7940.9134 Da.

1   GLYPROLYSTHRLEUHISGLULEULEUGLU
2   ARGILEGLYLEUGLUGLUHISTHRSERTHR
3   LEULEULEUASNGLYTYRGLNTHRLEUGLU
4   ASPPHELYSGLULEUARGGLUTHRHISLEU
5   ASNGLULEUASNILEMETASPPROGLNHIS
6   ARGALALYSLEULEUTHRALAALAGLULEU
7   LEULEUASPTYRASPTHRGLYCYSGLU

Related Database Links:

UNP Q8K352
AlphaFold Q9DBV2

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