BMRB Entry 27425

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR27425

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Title:
1/loop partially truncated Phosphomimetic Bcl-2 mutant
Deposition date:
2018-03-14
Original release date:
2019-03-29
Authors:
Song, Ting; Xue, Zhenyu; Zhang, Zhichao
Citation:

Citation: Song, Ting; Cao, Keke; Fan, Yudan; Zhang, Zhichao; Guo, Zongwei; Zhang, Minhang; Liu, Peng. "Expression and solution NMR study of multi-site phosphomimetic mutant BCL-2 protein."  Protein Pept. Lett. 26, 449-457 (2019).
PubMed: 30919764

Assembly members:

Assembly members:
EEE-Bcl-2, polymer, 207 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET-28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EEE-Bcl-2: MAHAGRTGYDNREIVMKYIH YKLSQRGYEWDAGDVGAAPP GAAPAPGIFSSQPGHTPHPA ASRDPVARTSPLQTPAAPGA AAGPALSPVPPVVHLTLRQA GDDFSRRYRRDFAEMSSQLH LTPFTARGRFATVVEELFRD GVNWGRIVAFFEFGGVMCVE SVNREMSPLVDNIALWMTEY LNRHLHTWIQDNGGWDAFVE LYGPSMR

Data sets:
Data typeCount
13C chemical shifts83
15N chemical shifts78
1H chemical shifts78

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1EEE-Bcl-21

Entities:

Entity 1, EEE-Bcl-2 207 residues - Formula weight is not available

1   METALAHISALAGLYARGTHRGLYTYRASP
2   ASNARGGLUILEVALMETLYSTYRILEHIS
3   TYRLYSLEUSERGLNARGGLYTYRGLUTRP
4   ASPALAGLYASPVALGLYALAALAPROPRO
5   GLYALAALAPROALAPROGLYILEPHESER
6   SERGLNPROGLYHISTHRPROHISPROALA
7   ALASERARGASPPROVALALAARGTHRSER
8   PROLEUGLNTHRPROALAALAPROGLYALA
9   ALAALAGLYPROALALEUSERPROVALPRO
10   PROVALVALHISLEUTHRLEUARGGLNALA
11   GLYASPASPPHESERARGARGTYRARGARG
12   ASPPHEALAGLUMETSERSERGLNLEUHIS
13   LEUTHRPROPHETHRALAARGGLYARGPHE
14   ALATHRVALVALGLUGLULEUPHEARGASP
15   GLYVALASNTRPGLYARGILEVALALAPHE
16   PHEGLUPHEGLYGLYVALMETCYSVALGLU
17   SERVALASNARGGLUMETSERPROLEUVAL
18   ASPASNILEALALEUTRPMETTHRGLUTYR
19   LEUASNARGHISLEUHISTHRTRPILEGLN
20   ASPASNGLYGLYTRPASPALAPHEVALGLU
21   LEUTYRGLYPROSERMETARG

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SPARKY: Backbone or all simulated peaks