BMRB Entry 27400

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27400

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Title:
Assignment of 1H, 13C and 15N resonances of the extrecclular domain of human Death Receptor DR5
Deposition date:
2018-02-09
Original release date:
2018-06-20
Authors:
Odaert, Benoit; Baudin, Antoine; Berbon, Melanie; Mackereth, Cameron; Guichard, Gilles
Citation:

Citation: Baudin, Antoine; Guichard, Anne; Collie, Gavin; Rousseau, Sabrina; Chaignepain, Stephane; Hocquellet, Agnes; Berbon, Melanie; Loquet, Antoine; Mackereth, Cameron; Guichard, Gilles; Odaert, Benoit. "1H, 13C, 15N NMR resonance assignments and secondary structure determination of the extra-cellular domain from the human proapoptotic TRAIL-R2 death receptor 5 (DR5-ECD)"  Biomol. NMR Assignments 12, 309-314 (2018).
PubMed: 29869749

Assembly members:

Assembly members:
DR5, polymer, 135 residues, 14998 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETNus_1a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DR5: GAMESALITQQDLAPQQRAA PQQKRSSPSEGLCPPGHHIS EDGRDCISCKYGQDYSTHWN DLLFCLRCTRCDSGEVELSP CTTTRNTVCQCEEGTFREED SPEMCRKCRTGCPRGMVKVG DCTPWSDIECVHKES

Data sets:
Data typeCount
13C chemical shifts447
15N chemical shifts114
1H chemical shifts653

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DR5 monomer1

Entities:

Entity 1, DR5 monomer 135 residues - 14998 Da.

Residus 1-4 represent a non-native cleavage site for the TeV protease

1   GLYALAMETGLUSERALALEUILETHRGLN
2   GLNASPLEUALAPROGLNGLNARGALAALA
3   PROGLNGLNLYSARGSERSERPROSERGLU
4   GLYLEUCYSPROPROGLYHISHISILESER
5   GLUASPGLYARGASPCYSILESERCYSLYS
6   TYRGLYGLNASPTYRSERTHRHISTRPASN
7   ASPLEULEUPHECYSLEUARGCYSTHRARG
8   CYSASPSERGLYGLUVALGLULEUSERPRO
9   CYSTHRTHRTHRARGASNTHRVALCYSGLN
10   CYSGLUGLUGLYTHRPHEARGGLUGLUASP
11   SERPROGLUMETCYSARGLYSCYSARGTHR
12   GLYCYSPROARGGLYMETVALLYSVALGLY
13   ASPCYSTHRPROTRPSERASPILEGLUCYS
14   VALHISLYSGLUSER

Related Database Links:

GB AAC01565.1

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