BMRB Entry 27395

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27395

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Title:
Backbone and methyl assignments for human Hsc70 substrate binding domain
Deposition date:
2018-01-31
Original release date:
2018-05-09
Authors:
Zuiderweg, Erik; Morshauser, Robert
Citation:

Citation: Taylor, Isabelle; Ahmad, Atta; Wu, T.; Nordhues, Bryce; Bhullar, Anup; Gestwicki, Jason; Zuiderweg, Erik. "The disorderly conduct of Hsc70 and its interaction with the Alzheimer's related Tau protein"  J. Biol. Chem. 293, 10796-10809 (2018).
PubMed: 29764935

Assembly members:

Assembly members:
Hsc70-SBD, polymer, 166 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hsc70-SBD: MSNADVTPLSLGIETAGGVM TVLIKRNTTIPTKQTQTFTT YSDNQPGVLIQVYEGERAMT KDNNLLGKFELTGIPPAPRG VPQIEVTFDIDANGILNVSA VDKSTGKENKITITNDKGRL SKEDIERMVQEAEKYKAEDE KQQPGGGKVQIINKKLDGGG HHHHHH

Data sets:
Data typeCount
13C chemical shifts460
15N chemical shifts122
1H chemical shifts362

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Hsc701

Entities:

Entity 1, Hsc70 166 residues - Formula weight is not available

391-394 MSNA cloning artifact 395-532 HSC70 sequence 533-547 TAU sequence QPGGGKVQIINKKLDG 548-556 GGGHHHHHH HIS TAG

1   METSERASNALAASPVALTHRPROLEUSER
2   LEUGLYILEGLUTHRALAGLYGLYVALMET
3   THRVALLEUILELYSARGASNTHRTHRILE
4   PROTHRLYSGLNTHRGLNTHRPHETHRTHR
5   TYRSERASPASNGLNPROGLYVALLEUILE
6   GLNVALTYRGLUGLYGLUARGALAMETTHR
7   LYSASPASNASNLEULEUGLYLYSPHEGLU
8   LEUTHRGLYILEPROPROALAPROARGGLY
9   VALPROGLNILEGLUVALTHRPHEASPILE
10   ASPALAASNGLYILELEUASNVALSERALA
11   VALASPLYSSERTHRGLYLYSGLUASNLYS
12   ILETHRILETHRASNASPLYSGLYARGLEU
13   SERLYSGLUASPILEGLUARGMETVALGLN
14   GLUALAGLULYSTYRLYSALAGLUASPGLU
15   LYSGLNGLNPROGLYGLYGLYLYSVALGLN
16   ILEILEASNLYSLYSLEUASPGLYGLYGLY
17   HISHISHISHISHISHIS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks