BMRB Entry 27248

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27248

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Title:
Backbone amide and AILV methyl chemical shift assignments for Beta2-microglobulin, a human class I major histocompatibility molecule light chain
Deposition date:
2017-09-08
Original release date:
2018-08-16
Authors:
Sgourakis, Nikolaos; McShan, Andrew; Natarajan, Kannan; Kumirov, Vlad; Margulies, David; Nerli, Santrupti; Badstuebner, Mareike
Citation:

Citation: McShan, Andrew; Natarajan, Kannan; Kumirov, Vlad; Flores-Solis, David; Jiang, Jiansheng; Badstubner, Mareike; Toor, Jugmohit; Bagshaw, Clive; Kovrigin, Evgenii; Margulies, David; Sgourakis, Nikolaos. "Peptide exchange on MHC-I by TAPBPR is driven by a negative allostery release cycle"  Nat. Chem. Biol. 14, 811-820 (2018).
PubMed: 29988068

Assembly members:

Assembly members:
H2-Dd, polymer, 277 residues, Formula weight is not available
Beta-2_microglobulin, polymer, 100 residues, Formula weight is not available
P18-I10, polymer, 10 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: HIV-1   Taxonomy ID: 11676   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus HIV-1

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
H2-Dd: MSHSLRYFVTAVSRPGFGEP RYMEVGYVDNTEFVRFDSDA ENPRYEPRARWIEQEGPEYW ERETRRAKGNEQSFRVDLRT ALRYYNQSAGGSHTLQWMAG CDVESDGRLLRGYWQFAYDG CDYIALNEDLKTWTAADMAA QITRRKWEQAGAAERDRAYL EGECVEWLRRYLKNGNATLL RTDPPKAHVTHHRRPEGDVT LRCWALGFYPADITLTWQLN GEELTQEMELVETRPAGDGT FQKWASVVVPLGKEQKYTCH VEHEGLPEPLTLRWGKE
Beta-2_microglobulin: MIQRTPKIQVYSRHPAENGK SNFLNCYVSGFHPSDIEVDL LKNGERIEKVEHSDLSFSKD WSFYLLYYTEFTPTEKDEYA CRVNHVTLSQPKIVKWDRDM
P18-I10: RGPGRAFVTI

Data sets:
Data typeCount
13C chemical shifts219
15N chemical shifts90
1H chemical shifts195

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Antigen peptide3
2Heavy chain1
3Light chain2

Entities:

Entity 3, Antigen peptide 10 residues - Formula weight is not available

1   ARGGLYPROGLYARGALAPHEVALTHRILE

Entity 1, Heavy chain 277 residues - Formula weight is not available

1   METSERHISSERLEUARGTYRPHEVALTHR
2   ALAVALSERARGPROGLYPHEGLYGLUPRO
3   ARGTYRMETGLUVALGLYTYRVALASPASN
4   THRGLUPHEVALARGPHEASPSERASPALA
5   GLUASNPROARGTYRGLUPROARGALAARG
6   TRPILEGLUGLNGLUGLYPROGLUTYRTRP
7   GLUARGGLUTHRARGARGALALYSGLYASN
8   GLUGLNSERPHEARGVALASPLEUARGTHR
9   ALALEUARGTYRTYRASNGLNSERALAGLY
10   GLYSERHISTHRLEUGLNTRPMETALAGLY
11   CYSASPVALGLUSERASPGLYARGLEULEU
12   ARGGLYTYRTRPGLNPHEALATYRASPGLY
13   CYSASPTYRILEALALEUASNGLUASPLEU
14   LYSTHRTRPTHRALAALAASPMETALAALA
15   GLNILETHRARGARGLYSTRPGLUGLNALA
16   GLYALAALAGLUARGASPARGALATYRLEU
17   GLUGLYGLUCYSVALGLUTRPLEUARGARG
18   TYRLEULYSASNGLYASNALATHRLEULEU
19   ARGTHRASPPROPROLYSALAHISVALTHR
20   HISHISARGARGPROGLUGLYASPVALTHR
21   LEUARGCYSTRPALALEUGLYPHETYRPRO
22   ALAASPILETHRLEUTHRTRPGLNLEUASN
23   GLYGLUGLULEUTHRGLNGLUMETGLULEU
24   VALGLUTHRARGPROALAGLYASPGLYTHR
25   PHEGLNLYSTRPALASERVALVALVALPRO
26   LEUGLYLYSGLUGLNLYSTYRTHRCYSHIS
27   VALGLUHISGLUGLYLEUPROGLUPROLEU
28   THRLEUARGTRPGLYLYSGLU

Entity 2, Light chain 100 residues - Formula weight is not available

1   METILEGLNARGTHRPROLYSILEGLNVAL
2   TYRSERARGHISPROALAGLUASNGLYLYS
3   SERASNPHELEUASNCYSTYRVALSERGLY
4   PHEHISPROSERASPILEGLUVALASPLEU
5   LEULYSASNGLYGLUARGILEGLULYSVAL
6   GLUHISSERASPLEUSERPHESERLYSASP
7   TRPSERPHETYRLEULEUTYRTYRTHRGLU
8   PHETHRPROTHRGLULYSASPGLUTYRALA
9   CYSARGVALASNHISVALTHRLEUSERGLN
10   PROLYSILEVALLYSTRPASPARGASPMET

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks