BMRB Entry 27246

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27246

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Title:
Backbone assignments of engineered hairpin loop3 mutant of single chain monellin.
Deposition date:
2017-09-08
Original release date:
2019-02-08
Authors:
Surana, Parag; Nandwani, Neha; Udgaonkar, Jayant; Gosavi, Shachi; Das, Ranabir
Citation:

Citation: Nandwani, Neha; Surana, Parag; Negi, Hitendra; Mascarenhas, Nahren; Udgaonkar, Jayant; Das, Ranabir; Gosavi, Shachi. "A five-residue motif for the design of domain swapping in proteins."  Nat. Commun. 10, 452-452 (2019).
PubMed: 30692525

Assembly members:

Assembly members:
single_chain_monellin, polymer, 100 residues, 11479 Da.

Natural source:

Natural source:   Common Name: serendipity berry   Taxonomy ID: 3457   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Dioscoreophyllum cumminsi

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
single_chain_monellin: MGEWEIIDIGPFTQNLGKFA VDEENKIGQYGRLTFNKVIR PCMKKTIYENEGFREIKGYE YQLYVYASDKLFRADISEQV VAGGRKLLRFNGPVPPPSTP

Data sets:
Data typeCount
13C chemical shifts263
15N chemical shifts89
1H chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1single chain monellin1

Entities:

Entity 1, single chain monellin 100 residues - 11479 Da.

1   METGLYGLUTRPGLUILEILEASPILEGLY
2   PROPHETHRGLNASNLEUGLYLYSPHEALA
3   VALASPGLUGLUASNLYSILEGLYGLNTYR
4   GLYARGLEUTHRPHEASNLYSVALILEARG
5   PROCYSMETLYSLYSTHRILETYRGLUASN
6   GLUGLYPHEARGGLUILELYSGLYTYRGLU
7   TYRGLNLEUTYRVALTYRALASERASPLYS
8   LEUPHEARGALAASPILESERGLUGLNVAL
9   VALALAGLYGLYARGLYSLEULEUARGPHE
10   ASNGLYPROVALPROPROPROSERTHRPRO

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks