BMRB Entry 27192

Name: NMR assignment of Rab5a (double deletion mutant) from Leishmania donovani
Published: 2019-01-10

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27192

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3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR assignment of Rab5a (double deletion mutant) from Leishmania donovani

Deposition date:

2017-07-22

Original release date:

2019-01-10

Authors:

Maheshwari, Diva; Yadav, Rahul; Mukhopadhyay, Amitabha; Arora, Ashish

Citation:

Citation: Maheshwari, Diva; Yadav, Rahul; Rastogi, Ruchir; Jain, Anupam; Tripathi, Sarita; Mukhopadhyay, Amitabha; Arora, Ashish. "Structural and Biophysical Characterization of Rab5a from Leishmania Donovani" Biophys. J. 115, 1217-1230 (2018).
PubMed: 30241678

Assembly members:

Assembly members:
Rab5a, polymer, 175 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Leishmania donovani Taxonomy ID: 5661 Superkingdom: Eukaryota Kingdom: Excavata Genus/species: Leishmania donovani

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rab5a: MNTHPPQLMEATSAKIVMLG ESGAGKSSIALRFTRNEFLA NQETTIGAAFLSKTVMIDGR ALKYEIWDTAGLERFRSLAP IYYRGASGALVVYDITNSES LKKAQTWIKELRANADPSLI IVLVGNKKDLGSLRQVSFED GQRLAAEEQLAAFYEASAKD NNNVEQVFLDLAAKL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	632
15N chemical shifts	165
1H chemical shifts	1024

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Rab5a	1

Entities:

Entity 1, Rab5a 175 residues - Formula weight is not available

1

MET

ASN

THR

HIS

PRO

GLN

LEU

MET

GLU

2

ALA

THR

SER

ALA

LYS

ILE

VAL

MET

LEU

GLY

3

GLU

SER

GLY

ALA

GLY

LYS

SER

ILE

ALA

4

LEU

ARG

PHE

THR

ARG

ASN

GLU

PHE

LEU

ALA

5

ASN

GLN

GLU

THR

ILE

GLY

ALA

PHE

6

LEU

SER

LYS

THR

VAL

MET

ILE

ASP

GLY

ARG

7

ALA

LEU

LYS

TYR

GLU

ILE

TRP

ASP

THR

ALA

8

GLY

LEU

GLU

ARG

PHE

ARG

SER

LEU

ALA

PRO

9

ILE

TYR

ARG

GLY

ALA

SER

GLY

ALA

LEU

10

VAL

TYR

ASP

ILE

THR

ASN

SER

GLU

SER

11

LEU

LYS

ALA

GLN

THR

TRP

ILE

LYS

GLU

12

LEU

ARG

ALA

ASN

ALA

ASP

PRO

SER

LEU

ILE

13

ILE

VAL

LEU

VAL

GLY

ASN

LYS

ASP

LEU

14

GLY

SER

LEU

ARG

GLN

VAL

SER

PHE

GLU

ASP

15

GLY

GLN

ARG

LEU

ALA

GLU

GLN

LEU

16

ALA

PHE

TYR

GLU

ALA

SER

ALA

LYS

ASP

17

ASN

VAL

GLU

GLN

VAL

PHE

LEU

ASP

18

LEU

ALA

LYS

LEU

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HN(CA)CO	sample_2	isotropic	sample_conditions_1
3D H(CCO)NH	sample_2	isotropic	sample_conditions_1
3D C(CO)NH	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 27192

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers: