BMRB Entry 27169

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27169

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Title:
1H, 13C, and 15N Chemical Shift Assignments for truncated forms of the coronavirus nsp1 protein and its solubility domain GB1
Deposition date:
2017-07-06
Original release date:
2017-08-10
Authors:
Vazquez, Leonardo; Almeida, Marcius
Citation:

Citation: Vazquez, Leonardo; Lima, Luis; Almeida, Marcius. "Comprehensive structural analysis of designed incomplete polypeptide chains of the replicase nonstructural protein 1 from the severe acute respiratory syndrome coronavirus"  PLoS ONE 12, e0182132-e0182132 (2017).
PubMed: 28750053

Assembly members:

Assembly members:
GB1, polymer, 86 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS coronavirus   Taxonomy ID: 227859   Superkingdom: Viruses   Kingdom: not available   Genus/species: SARS coronavirus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-25(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GB1: MFQYKLILNGKTLKGETTTE AVDAATAEKVFKQYANDNGV DGEWTYDDATKTFTVTEGSA TMAENLYFQGLSLANAAALE HHHHHH

Data sets:
Data typeCount
13C chemical shifts244
15N chemical shifts65
1H chemical shifts234

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1solubility domain GB11

Entities:

Entity 1, solubility domain GB1 86 residues - Formula weight is not available

1   METPHEGLNTYRLYSLEUILELEUASNGLY
2   LYSTHRLEULYSGLYGLUTHRTHRTHRGLU
3   ALAVALASPALAALATHRALAGLULYSVAL
4   PHELYSGLNTYRALAASNASPASNGLYVAL
5   ASPGLYGLUTRPTHRTYRASPASPALATHR
6   LYSTHRPHETHRVALTHRGLUGLYSERALA
7   THRMETALAGLUASNLEUTYRPHEGLNGLY
8   LEUSERLEUALAASNALAALAALALEUGLU
9   HISHISHISHISHISHIS

Download HSQC peak lists in one of the following formats:
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