BMRB Entry 27133
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27133
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NMR-STAR v3 text file.
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Citation: Buchinger, Edith; Wiik, Siv; Kusnierczyk, Anna; Rabe, Renana; Aas, Per; Kavli, Bodil; Slupphaug, Geir; Aachmann, Finn. "Backbone 1H, 13C and 15N chemical shift assignment of full-length human uracil DNA glycosylase UNG2" Biomol. NMR Assign. 12, 15-22 (2018).
PubMed: 28879561
Assembly members:
NUNG2, polymer, 119 residues, Formula weight is not available
CUNG2, polymer, 246 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTYB12 and pVB-1A0B1
Entity Sequences (FASTA):
NUNG2: MIGQKTLYSFFSPSPARKRH
APSPEPAVQGTGVAGVPEES
GDAAAIPAKKAPAGQEEPGT
PPSSPLSAEQLDRIQRNKAA
ALLRLAARNVPVAAAGSEQK
LISEEDLKASGADHHHHHH
CUNG2: MGSSHHHHHHSSGLVPRGSH
MIEGRCFGESWKKHLSGEFG
KPYFIKLMGFVAEERKHYTV
YPPPHQVFTWTQMCDIKDVK
VVILGQDPYHGPNQAHGLCF
SVQRPVPPPPSLENIYKELS
TDIEDFVHPGHGDLSGWAKQ
GVLLLNAVLTVRAHQANSHK
ERGWEQFTDAVVSWLNQNSN
GLVFLLWGSYAQKKGSAIDR
KRHHVLQTAHPSPLSVYRGF
FGCRHFSKTNELLQKSGKKP
IDWKEL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 1355 |
| 15N chemical shifts | 516 |
| 1H chemical shifts | 1303 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | N-terminal UNG2 | 1 |
| 2 | C-terminal UNG2 | 2 |
Entities:
Entity 1, N-terminal UNG2 119 residues - Formula weight is not available
A93-H119 is a non-native purification tag that was not included in this upload
| 1 | MET | ILE | GLY | GLN | LYS | THR | LEU | TYR | SER | PHE | ||||
| 2 | PHE | SER | PRO | SER | PRO | ALA | ARG | LYS | ARG | HIS | ||||
| 3 | ALA | PRO | SER | PRO | GLU | PRO | ALA | VAL | GLN | GLY | ||||
| 4 | THR | GLY | VAL | ALA | GLY | VAL | PRO | GLU | GLU | SER | ||||
| 5 | GLY | ASP | ALA | ALA | ALA | ILE | PRO | ALA | LYS | LYS | ||||
| 6 | ALA | PRO | ALA | GLY | GLN | GLU | GLU | PRO | GLY | THR | ||||
| 7 | PRO | PRO | SER | SER | PRO | LEU | SER | ALA | GLU | GLN | ||||
| 8 | LEU | ASP | ARG | ILE | GLN | ARG | ASN | LYS | ALA | ALA | ||||
| 9 | ALA | LEU | LEU | ARG | LEU | ALA | ALA | ARG | ASN | VAL | ||||
| 10 | PRO | VAL | ALA | ALA | ALA | GLY | SER | GLU | GLN | LYS | ||||
| 11 | LEU | ILE | SER | GLU | GLU | ASP | LEU | LYS | ALA | SER | ||||
| 12 | GLY | ALA | ASP | HIS | HIS | HIS | HIS | HIS | HIS |
Entity 2, C-terminal UNG2 246 residues - Formula weight is not available
The first 25AA are a non-native purification tag followed by a C26G mutant.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | ILE | GLU | GLY | ARG | CYS | PHE | GLY | GLU | SER | ||||
| 4 | TRP | LYS | LYS | HIS | LEU | SER | GLY | GLU | PHE | GLY | ||||
| 5 | LYS | PRO | TYR | PHE | ILE | LYS | LEU | MET | GLY | PHE | ||||
| 6 | VAL | ALA | GLU | GLU | ARG | LYS | HIS | TYR | THR | VAL | ||||
| 7 | TYR | PRO | PRO | PRO | HIS | GLN | VAL | PHE | THR | TRP | ||||
| 8 | THR | GLN | MET | CYS | ASP | ILE | LYS | ASP | VAL | LYS | ||||
| 9 | VAL | VAL | ILE | LEU | GLY | GLN | ASP | PRO | TYR | HIS | ||||
| 10 | GLY | PRO | ASN | GLN | ALA | HIS | GLY | LEU | CYS | PHE | ||||
| 11 | SER | VAL | GLN | ARG | PRO | VAL | PRO | PRO | PRO | PRO | ||||
| 12 | SER | LEU | GLU | ASN | ILE | TYR | LYS | GLU | LEU | SER | ||||
| 13 | THR | ASP | ILE | GLU | ASP | PHE | VAL | HIS | PRO | GLY | ||||
| 14 | HIS | GLY | ASP | LEU | SER | GLY | TRP | ALA | LYS | GLN | ||||
| 15 | GLY | VAL | LEU | LEU | LEU | ASN | ALA | VAL | LEU | THR | ||||
| 16 | VAL | ARG | ALA | HIS | GLN | ALA | ASN | SER | HIS | LYS | ||||
| 17 | GLU | ARG | GLY | TRP | GLU | GLN | PHE | THR | ASP | ALA | ||||
| 18 | VAL | VAL | SER | TRP | LEU | ASN | GLN | ASN | SER | ASN | ||||
| 19 | GLY | LEU | VAL | PHE | LEU | LEU | TRP | GLY | SER | TYR | ||||
| 20 | ALA | GLN | LYS | LYS | GLY | SER | ALA | ILE | ASP | ARG | ||||
| 21 | LYS | ARG | HIS | HIS | VAL | LEU | GLN | THR | ALA | HIS | ||||
| 22 | PRO | SER | PRO | LEU | SER | VAL | TYR | ARG | GLY | PHE | ||||
| 23 | PHE | GLY | CYS | ARG | HIS | PHE | SER | LYS | THR | ASN | ||||
| 24 | GLU | LEU | LEU | GLN | LYS | SER | GLY | LYS | LYS | PRO | ||||
| 25 | ILE | ASP | TRP | LYS | GLU | LEU |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks