BMRB Entry 27081

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PDB ID: 5wlp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27081
MolProbity Validation Chart

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Title:
1H, 13C, and 15N backbone and side chain resonance assignments for a structured domain in atg32
Deposition date:
2017-04-22
Original release date:
2017-11-16
Authors:
Xia, Xue; Pellegrini, Maria; Ragusa, Michael
Citation:

Citation: Xia, Xue; Pellegrini, Maria; Ragusa, Michael. "Backbone and side chain resonance assignments for a structured domain within Atg32"  Biomol. NMR Assignments 11, 211-214 (2017).
PubMed: 28766175

Assembly members:

Assembly members:
atg32, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET His6 TEV LIC cloning vector (1B)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
atg32: SNATNSFVMPKLSLTQKNPV FRLLILGRTGSSFYQSIPKE YQSLFELPKYHDSATFPQYT GIVIIFQELREMVSLLNRIV QYSQGKPVIPIcQPGQVIQV KNVLKSFLRNKLVKLLFPPV VVTNKRDLKKMFQRLQDLSL EYGED

Data sets:
Data typeCount
13C chemical shifts603
15N chemical shifts140
1H chemical shifts1040

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1atg321

Entities:

Entity 1, atg32 145 residues - Formula weight is not available

1   SERASNALATHRASNSERPHEVALMETPRO
2   LYSLEUSERLEUTHRGLNLYSASNPROVAL
3   PHEARGLEULEUILELEUGLYARGTHRGLY
4   SERSERPHETYRGLNSERILEPROLYSGLU
5   TYRGLNSERLEUPHEGLULEUPROLYSTYR
6   HISASPSERALATHRPHEPROGLNTYRTHR
7   GLYILEVALILEILEPHEGLNGLULEUARG
8   GLUMETVALSERLEULEUASNARGILEVAL
9   GLNTYRSERGLNGLYLYSPROVALILEPRO
10   ILECYSGLNPROGLYGLNVALILEGLNVAL
11   LYSASNVALLEULYSSERPHELEUARGASN
12   LYSLEUVALLYSLEULEUPHEPROPROVAL
13   VALVALTHRASNLYSARGASPLEULYSLYS
14   METPHEGLNARGLEUGLNASPLEUSERLEU
15   GLUTYRGLYGLUASP

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks